Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 7, 2016; 22(33): 7536-7558
Published online Sep 7, 2016. doi: 10.3748/wjg.v22.i33.7536
Impact of Helicobacter pylori on the healing process of the gastric barrier
Eliza Mnich, Magdalena Kowalewicz-Kulbat, Paulina Sicińska, Krzysztof Hinc, Michał Obuchowski, Adrian Gajewski, Anthony P Moran, Magdalena Chmiela
Eliza Mnich, Magdalena Kowalewicz-Kulbat, Adrian Gajewski, Magdalena Chmiela, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Łódź, 90-237 Łódź, Poland
Paulina Sicińska, Department of Biophysics of Environment Pollution, Faculty of Biology and Environmental Protection, University of Łódź, 90-237 Łódź, Poland
Krzysztof Hinc, Michał Obuchowski, Laboratory of Molecular Bacteriology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, 80-210 Gdańsk, Poland
Anthony P Moran, Department of Microbiology, Laboratory of Molecular Biochemistry, University College, 9014 Galway, Ireland
Author contributions: Mnich E, Chmiela M substantially contributed to the conception and design of the research, performed analysis and data interpretation; Mnich E participated in all experiments; Kowalewicz-Kulbat M and Sicińska P performed the cell cycle and apoptosis assay; Gajewski A participated in DAPI staining; Hinc K, Obuchowski M and Moran AP provided essential reagents: subunit A of urease, H. pylori LPS; Mnich E and Kowalewicz-Kulbat M performed statistical analysis; all authors drafted the article and made critical revisions related to the intellectual content of the manuscript, and approved the final version of the article to be published.
Supported by National Science Centre of Poland, No. DEC-2013/09/N/NZ6/00805 and No. DEC-2015/17/N/NZ6/03490.
Conflict-of-interest statement: The authors have no competing interests.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Magdalena Chmiela, Professor, PhD, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. chmiela@biol.uni.lodz.pl
Telephone: +48-42-6354186 Fax: +48-42-6655818
Received: May 7, 2016
Peer-review started: May 8, 2016
First decision: May 20, 2016
Revised: June 29, 2016
Accepted: July 20, 2016
Article in press: July 20, 2016
Published online: September 7, 2016
Processing time: 120 Days and 13.2 Hours
Core Tip

Core tip: This manuscript focused on the impact of Helicobacter pylori (H. pylori) antigens to the gastric mucosal barrier. We evaluated the effects of H. pylori antigens using in vitro two cellular models of gastric epithelial cells and fibroblasts, which had been independently exposed to H. pylori components. In this study, we showed different effects of subunit A of urease, cytotoxin associated gene A protein, lipopolysaccharide (LPS) as well as compounds included in a glycine acid extract on the regenerative activity of gastric epithelial cells and fibroblasts. Our results indicate deleterious, dose dependent influence of H. pylori LPS on this process.