Basic Study
Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 7, 2015; 21(9): 2638-2644
Published online Mar 7, 2015. doi: 10.3748/wjg.v21.i9.2638
Effect of bifidobacterium on defensin-5 expression in intestinal injury of preweaning rats
Wei Wang, Shu-Fen Yang, Li-Hong Ren, Xiu-Xiu Zhang, Shui-Lian Yu
Wei Wang, Shu-Fen Yang, Li-Hong Ren, Xiu-Xiu Zhang, Shui-Lian Yu, Department of Pediatrics, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Author contributions: Wang W designed and performed the research, analyzed the data and wrote the paper; Yang SF designed and performed the research; Ren LH designed the research and analyzed the data; Zhang XX and Yu SL contributed new reagents or analytic tools.
Supported by Foundation of Education Department of Heilongjiang Province, No. 11521124.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Wei Wang, MD, Department of Pediatrics, the Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu Road, Nangang District, Harbin 150086, Heilongjiang Province, China. wendywang_1972@126.com
Telephone: +86-451-86605665 Fax: +86-451-86605665
Received: September 21, 2014
Peer-review started: September 21, 2014
First decision: October 14, 2014
Revised: October 29, 2014
Accepted: December 5, 2014
Article in press: December 8, 2014
Published online: March 7, 2015
Processing time: 169 Days and 7.2 Hours
Core Tip

Core tip: This study investigated the serum and ileal contents of myeloperoxidase (MPO) and the expression of ileal Defensin-5 mRNA in the preweaning rats with lipopolysaccharide (LPS)-induced intestinal injury. Serum and ileal MPO contents in the model group (group E) were significantly higher than those in the control group (group C) (P < 0.05), which peaked at 12 h and 6 h, respectively. MPO contents in the treatment group (group T) were significantly lower than those in group E (P < 0.05). The expression of defensin-5 mRNA in group E was significantly up-regulated, peaked at 2 h, and then decreased gradually. The expression of defensin-5 mRNA in group T was significantly lower than that in group E (P < 0.05). Serum and ileal MPO contents and ileal expression of defensin-5 mRNA increased in preweaning rats with LPS-induced intestinal injury. Bifidobacterium protects the gut by inhibiting MPO activity, not by increasing defensin-5 secretion.