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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 7, 2015; 21(21): 6526-6542
Published online Jun 7, 2015. doi: 10.3748/wjg.v21.i21.6526
Published online Jun 7, 2015. doi: 10.3748/wjg.v21.i21.6526
New NCI-N87-derived human gastric epithelial line after human telomerase catalytic subunit over-expression
Kathy Saraiva-Pava, Faculdade de Engenharia, Universidade Católica Portuguesa, Campus de Sintra, 2635-631 Rio de Mouro, Portugal
Nazanin Navabi, Emma C Skoog, Sara K Lindén, Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, SE-40530 Gothenburg, Sweden
Mónica Oleastro, Departamento de Doenças Infeciosas, Instituto Nacional Saúde Dr. Ricardo Jorge, 1649-016 Lisboa, Portugal
Mónica Roxo-Rosa, Chronic Diseases Research Center, CEDOC, Nova Medical School, Campo Mártires da Pátria, 1169-056 Lisboa, Portugal
Author contributions: Lindén SK, Oleastro M and Roxo-Rosa M designed the research; Saraiva-Pava K, Navabi N and Skoog EC performed the research; Roxo-Rosa M, Lindén SK and Oleastro M analyzed the data; Saraiva-Pava K, Lindén SK and Roxo-Rosa M wrote the paper.
Supported by Grants from the Fundação para a Ciência e a Tecnologia (FCT, Portugal), No. PPCDT/SAL-IMI/57297/2004 and No. PTDC/BIM-MEC/1051/2012; The Swedish Cancer foundation; The Swedish Research Council, No. K2010-79X-21372-01-3; Forska utan djurförsök, Animal Free Research; and by Research fellowship 2011 from the Sociedade Portuguesa de Gastrenterologia (Portugal).
Conflict-of-interest: The authors have no competing interests.
Data sharing: Technical appendix, statistical code, and dataset available from the corresponding author at monica.oleastro@insa.min-saude.pt. Participants gave informed consent for data sharing.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Mónica Oleastro, PhD, Departamento de Doenças Infeciosas, Instituto Nacional Saúde Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisboa, Portugal. monica.oleastro@insa.min-saude.pt
Telephone: +351-217519200 Fax: +351-217526400
Received: October 14, 2014
Peer-review started: October 15, 2014
First decision: January 8, 2015
Revised: February 7, 2015
Accepted: March 31, 2015
Article in press: March 31, 2015
Published online: June 7, 2015
Processing time: 239 Days and 14 Hours
Peer-review started: October 15, 2014
First decision: January 8, 2015
Revised: February 7, 2015
Accepted: March 31, 2015
Article in press: March 31, 2015
Published online: June 7, 2015
Processing time: 239 Days and 14 Hours
Core Tip
Core tip: In this study, we aimed to establish and characterize novel human gastric epithelial cell lines derived from NCI-N87 cells after over-expression of human telomerase catalytic activity. The two most promising NCI-N87-derived clones were shown to be composed of cells with homogenous phenotype, to produce gastric zymogens and to produce and secrete neutral mucins. In addition, these clones showed very good in vitro growth properties, resistance to microaerobic conditions and good responsiveness to Helicobacter pylori. Due to their improved properties, compared to the heterogeneous parental line, these NCI-N87-derived clones are promising models of the human gastric epithelium.