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World J Gastroenterol. Feb 28, 2014; 20(8): 2051-2061
Published online Feb 28, 2014. doi: 10.3748/wjg.v20.i8.2051
Published online Feb 28, 2014. doi: 10.3748/wjg.v20.i8.2051
Longitudinal analysis of inflammation and microbiota dynamics in a model of mild chronic dextran sulfate sodium-induced colitis in mice
Luigia De Fazio, Elena Cavazza, Enzo Spisni, Antonio Strillacci, Maria Chiara Valerii, Department of Biological, Geological and Environmental Sciences, Biology Unit, University of Bologna, Via Selmi 3, 40126 Bologna, Italy
Manuela Centanni, Marco Candela, Department of Pharmacy and Biotechnology, University of Bologna, Via 6, 40126 Bologna, Italy
Chiara Praticò, Massimo Campieri, Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy
Chiara Ricci, Department of Clinical and Experimental Sciences, University of Brescia, Spedali Civili 1, 25121 Brescia, Italy
Author contributions: De Fazio L, Cavazza E and Valerii C contributed substantially to the data acquisition; Spisni E drafted the article and revised it critically; Centanni M and Candela M performed the microbiota analysis and interpreted the data; Strillacci A performed the COX-2 expression analysis and interpreted the data; Valerii MC, Praticò C and Campieri M contributed to the study design; Ricci C contributed to the histological evaluation of colitis and the data acquisition; De Fazio L and Cavazza E contributed equally to this work.
Supported by Xeda international, France
Correspondence to: Enzo Spisni, Professor, Department of Biological, Geological and Environmental Sciences, Biology Unit, University of Bologna, Via Selmi 3, 40126 Bologna, Italy. enzo.spisni@unibo.it
Telephone: +39-51-2094147 Fax: +39-51-2094286
Received: July 15, 2013
Revised: November 6, 2013
Accepted: November 12, 2013
Published online: February 28, 2014
Revised: November 6, 2013
Accepted: November 12, 2013
Published online: February 28, 2014
Core Tip
Core tip: Experimental animal models of colitis are important for investigating the physiopathological mechanisms underlying inflammatory bowel disease (IBD) in humans. Murine dextran sulfate sodium colitis models have been widely adopted and validated as relevant models for the translation of mice data to human IBD. Nevertheless, it is clear that models characterized by mild intestinal damages are more accurate for studying the effects of therapeutic agents. In this study, we developed a reproducible mild chronic colitis model, which allows the evaluation of the intestinal repair processes, the modulation of systemic inflammation and the recovery of the gut microbiotic homeostasis.