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World J Gastroenterol. Dec 7, 2014; 20(45): 17163-17170
Published online Dec 7, 2014. doi: 10.3748/wjg.v20.i45.17163
Published online Dec 7, 2014. doi: 10.3748/wjg.v20.i45.17163
Circulating tumor cells in pancreatic cancer patients: Enrichment and cultivation
Vladimir Bobek, Katarina Kolostova, Department of Laboratory Genetics, University Hospital Kralovske Vinohrady, 10034 Prague, Czech Republic
Vladimir Bobek, Department of Histology and Embryology, Wroclaw Medical University, 50-367 Wrocław, Poland
Vladimir Bobek, 3rd Department of Surgery, First Faculty of Medicine Charles University in Prague and University Hospital Motol, 10034 Prague, Czech Republic
Robert Gurlich, Petra Eliasova, Department of Surgery, University Hospital Kralovske Vinohrady, 0034 Prague, Czech Republic
Author contributions: Bobek V and Kolostova K designed the study; Kolostova K and Eliasova P performed laboratory examination and collection of the data; Gurlich R collected the samples and analyzed clinical data; Bobek V and Kolostova K wrote the manuscripts; all authors co-authored the final text.
Supported by Research project “CTC in gastrointestinal cancer “awarded by League Against Cancer Prague, Czech Republic and grant of the Czech Ministry of Health, No. IGA NT14439-3/2013; and by the projects by Ministry of Helath Czech Republic, conceptual development of research organization, University Hospital Motol, Prague Czech Republic, No. 000 64203
Correspondence to: Vladimir Bobek MD, PhD, Department of Laboratory Genetics, University Hospital Kralovske Vinohrady, Srobarova 50, 10034 Prague, Czech Republic. vbobek@centrum.cz
Telephone: +42-26-716262 Fax: +42-26-716262
Received: February 17, 2014
Revised: May 20, 2014
Accepted: July 15, 2014
Published online: December 7, 2014
Revised: May 20, 2014
Accepted: July 15, 2014
Published online: December 7, 2014
Core Tip
Core tip: Circulating tumor cells role in the process of pancreatic cancer dissemination should be studied in the context of the disease management. The ability to in vitro culture pancreatic circulating tumor cells (CTCs) could potentially help with the development of innovative treatments and diagnostic technologies. We presented simple size-based separation device for the isolation of viable CTCs. The isolation process is gentle allowing the subsequent CTC-cultivation in vitro and is antibody independent.