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World J Gastroenterol. Dec 7, 2014; 20(45): 17084-17091
Published online Dec 7, 2014. doi: 10.3748/wjg.v20.i45.17084
Published online Dec 7, 2014. doi: 10.3748/wjg.v20.i45.17084
Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice
Mi Na Kim, Seoul National University Healthcare System Gangnam Center, Seoul National University Hospital, Seoul 135-984, South Korea
Seong-Joon Koh, Department of Internal Medicine, Seoul National University Boramae Hospital, Seoul National University College of Medicine, Seoul 157-707, South Korea
Jung Mogg Kim, Department of Microbiology, Hanyang University College of Medicine, Seoul 133-792, South Korea
Jong Pil Im, Hyun Chae Jung, Joo Sung Kim, Department of Internal Medicine, Liver Research Institute, Seoul National University College of Medicine, Seoul 110-744, South Korea
Author contributions: Kim MN and Kim JS conceived of and designed the study, and wrote the paper; Kim MN and Koh SJ performed the experiments; Kim MN, Kim JM, Im JP, Jung HC and Kim JS analyzed the data; all authors read and approved the final version of the paper.
Correspondence to: Joo Sung Kim, MD, PhD, Department of Internal Medicine, Liver Research Institute, Seoul National University College of Medicine, 103 Daehak-ro, Chongno-gu, Seoul 110-744, South Korea. jooskim@snu.ac.kr
Telephone: +82-2-7408112 Fax: +82-2-7436701
Received: May 17, 2014
Revised: June 27, 2014
Accepted: July 29, 2014
Published online: December 7, 2014
Processing time: 207 Days and 5 Hours
Revised: June 27, 2014
Accepted: July 29, 2014
Published online: December 7, 2014
Processing time: 207 Days and 5 Hours
Core Tip
Core tip: The results of this study indicate that Clostridium difficile (C. difficile) infection induces more severe colitis in the interleukin 10-deficient (IL-10-/-) mouse model of inflammatory bowel disease (IBD). Moreover, induction of interferon-γ gene expression was greater in the IL-10-/- mice and their bone marrow-derived dendritic cells compared to wild type mice following C. difficile infection and exposure to C. difficile toxin A, respectively. This study demonstrates the establishment of C. difficile-aggravated colitis in an IBD animal model, which provides a useful tool for studying the relationship between C. difficile and host immune response of the gut in IBD.