He YJ, Li WL, Liu BH, Dong H, Mou ZR, Wu YZ. Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester. World J Gastroenterol 2014; 20(33): 11840-11849 [PMID: 25206290 DOI: 10.3748/wjg.v20.i33.11840]
Corresponding Author of This Article
Zhi-Rong Mou, PhD, Institute of Immunology of PLA, the Third Military Medical University, Chongqing 400038, China. mouzr@yahoo.com
Research Domain of This Article
Oncology
Article-Type of This Article
Retrospective Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Yu-Jun He, Wan-Ling Li, Hui Dong, Zhi-Rong Mou, Yu-Zhang Wu, Institute of Immunology of PLA, the Third Military Medical University, Chongqing 400038, China
Yu-Jun He, Bao-Hua Liu, Department of General Surgery, Daping Hospital and Research Institute of Surgery, the Third Military Medical University, Chongqing 400042, China
Author contributions: He YJ and Mou ZR designed the research; He YJ, Li WL and Dong H performed the research; He YJ, Liu BH and Mou ZR analyzed the data; He YJ, Liu BH, Mou ZR and Wu YZ wrote the paper; Mou ZR and Wu YZ contributed equally to this work.
Supported by National Natural Science Foundation of China No. 30872466 and No. 30801096, the Natural Science Foundation of Chongqing No. 2011BB5032, and PLA Logistics Science Research during the 12th Five-Year Plan Period No. BWS11J041
Correspondence to: Zhi-Rong Mou, PhD, Institute of Immunology of PLA, the Third Military Medical University, Chongqing 400038, China. mouzr@yahoo.com
Telephone: +86-23-68752680 Fax: +86-23-68752789
Received: January 2, 2014 Revised: March 18, 2014 Accepted: April 28, 2014 Published online: September 7, 2014
Core Tip
Core tip: To investigate the molecular mechanisms of the anti-cancer activity of caffeic acid phenethyl ester (CAPE), CAPE-treated colorectal cancer SW480 cells were analysed by a 2D-gel based proteomics approach. Four up-regulated proteins and seven down-regulated proteins in CAPE-treated SW480 cells were found and further identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry following a database search. The down-regulated proteins, PSMA1 and PSAT1 and up-regulated proteins GNPDA1 and GPX-1 were validated by Western blotting. The two tumorigenesis associated proteins, PSMA1 and PSAT1, were further confirmed by immunofluorescence assay. These differentiated proteins in colorectal cancer cells following CAPE treatment, may be potential molecular targets of CAPE and involved in the anti-cancer effect of CAPE.
