Original Article
Copyright ©2014 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 28, 2014; 20(28): 9476-9485
Published online Jul 28, 2014. doi: 10.3748/wjg.v20.i28.9476
Establishment of an orthotopic pancreatic cancer mouse model: Cells suspended and injected in Matrigel
Yong-Jian Jiang, Chong-Lek Lee, Qiang Wang, Zhong-Wen Zhou, Feng Yang, Chen Jin, De-Liang Fu
Yong-Jian Jiang, Chong-Lek Lee, Qiang Wang, Feng Yang, Chen Jin, De-Liang Fu, Department of Pancreatic Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China
Zhong-Wen Zhou, Department of Pathology, Huashan Hospital, Fudan University, Shanghai 200040, China
Author contributions: Jiang YJ designed this study and wrote the first draft of the manuscript; Jiang YJ and Lee CL performed the experiments; Wang Q and Yang F analyzed the data; Zhou ZW reviewed all HE and immunohistochemical staining of the tumor specimens sections; Jin C and Fu DL critically reviewed the manuscript.
Supported by The Shanghai Municipal Natural Science Foundation, No. 11ZR1405500; and the Shanghai Municipal Science and Technology Commission grant, No. 13140902401
Correspondence to: De-Liang Fu, MD, PhD, Department of Pancreatic Surgery, Huashan Hospital, Fudan University, No. 12, Urumqi Middle Road, Shanghai 200040, China. surgeonfu@163.com
Telephone: +86-21-52887164 Fax: +86-21-52888277
Received: October 31, 2013
Revised: March 24, 2014
Accepted: April 5, 2014
Published online: July 28, 2014
Processing time: 267 Days and 16.2 Hours
Core Tip

Core tip: This article describes a simple and effective method to establish an orthotopic pancreatic cancer model in immunocompetent mice. The orthotopic pancreatic cancer models in the pancreatic head and tail both effectively mimic the relative pathological features of exocrine adenocarcinoma of the human pancreas. This model system will provide a platform for exploring new research outcomes for the effective treatment and management of pancreatic cancer.