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World J Gastroenterol. Jun 7, 2014; 20(21): 6651-6657
Published online Jun 7, 2014. doi: 10.3748/wjg.v20.i21.6651
Published online Jun 7, 2014. doi: 10.3748/wjg.v20.i21.6651
Novel method for extracting exosomes of hepatocellular carcinoma cells
Lin Zhu, Third School of Clinical Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China
Xiu-Hua Qu, Yang-Ming Qian, Xiao-Hang Zhao, Center of Basic Medical Sciences, Navy General Hospital of Chinese PLA, Beijing 100048, China
Yu-Lin Sun, Xiao-Hang Zhao, State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
Author contributions: Zhu L performed the majority of the work and wrote the paper; Qu XH and Sun YL assisted in the experiments and data analysis; Qian YM and Zhao XH were supervisors for this work; and Zhao XH designed the study.
Supported by Grants from the State Key Projects for Basic Research, No. 2011CB910703 and No. 2012ZX10002-017 (to Zhao XH); National High-tech R and D Program, No. 2013AA041201 (to Qian YM) and No. 2012AA020206 (to Zhao XH); National Natural Science Foundation of China, No. 81372591 and No. 81321091 (to Zhao XH); and the Research Foundation of the Center for Marine Medicine and Rescue of Tsinghua University and NGH (to Qian YM and Zhao XH)
Correspondence to: Xiao-Hang Zhao, Professor, Center of Basic Medical Sciences, Navy General Hospital of Chinese PLA, Beijing 100048, China. zhaoxh@cicams.ac.cn
Telephone: +86-10-66951482 Fax: +86-10-87778360
Received: October 31, 2013
Revised: February 8, 2014
Accepted: March 4, 2014
Published online: June 7, 2014
Processing time: 217 Days and 21.7 Hours
Revised: February 8, 2014
Accepted: March 4, 2014
Published online: June 7, 2014
Processing time: 217 Days and 21.7 Hours
Core Tip
Core tip: The traditional extraction assay of exosomes is usually complicated and time consuming. In this manuscript, we investigate a novel assay based on a nanomaterial to extract exosomes more quickly and efficiently compared with the traditional method. A hepatoma-associated protein, tissue transglutaminase 2, was first found to exist in the exosome of the human liver cancer cell line Huh-7. This protein can be secreted through an exosome-mediated non-classical secretion pathway and is a potential tumor marker.