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©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Dec 14, 2013; 19(46): 8630-8637
Published online Dec 14, 2013. doi: 10.3748/wjg.v19.i46.8630
Published online Dec 14, 2013. doi: 10.3748/wjg.v19.i46.8630
Clinicopathological and biological significance of cripto overexpression in human colon cancer
Peng-Cheng Jiang, Hao-Liang Zhao, Department of General Surgery, Affiliated First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Ling Zhu, Yu Fan, Cancer Institute, Affiliated People’s Hospital of Jiangsu University, Zhenjiang 212002, Jiangsu Province, China
Author contributions: Jiang PC, Zhu L and Fan Y performed the majority of experiments; Zhao HL and Jiang PC designed the study and wrote the manuscript.
Supported by The National Natural Science Fund, No. 81250035; The Jiangsu Province of 333 Engineering talent fund, No. RA2012103, and Zhenjiang City Social Development Fund No. SH2011031 and SH2011034
Correspondence to: Hao-Liang Zhao, Professor, Department of General Surgery, Affiliated First Hospital of Shanxi Medical University, The liberation of South, Taiyuan 030001, Shanxi Province, China. 18952819981@163.com
Telephone: +86-511-88917999 Fax: +86-511-85234387
Received: June 2, 2013
Revised: August 15, 2013
Accepted: September 29, 2013
Published online: December 14, 2013
Processing time: 198 Days and 15.2 Hours
Revised: August 15, 2013
Accepted: September 29, 2013
Published online: December 14, 2013
Processing time: 198 Days and 15.2 Hours
Core Tip
Core tip: To assess the clinicopathologic and biological significance of cripto as a novel target for colon cancer gene therapy, pathological and in vitro studies were carried out. Cripto was significantly overexpressed in primary colon cancer and metastatic lymph nodes. In vitro studies found that cripto siRNA resulted in a significant reduction in colony formation in soft agar and in the migration and invasion abilities of colon cancer cells. Furthermore, cripto siRNA led to an inhibition of MMP-7 and MMP-9. These results suggest that the cripto gene be useful for devising novel preventive and therapeutic strategies for colon cancer.