Hismiogullari AA, Hismiogullari SE, Rahman K. Isolation and biochemical analysis of vesicles from taurohyodeoxycholic acid-infused isolated perfused rat livers. World J Gastroenterol 2013; 19(37): 6228-6236 [PMID: 24115821 DOI: 10.3748/wjg.v19.i37.6228]
Corresponding Author of This Article
Dr. Adnan Adil Hismiogullari, Department of Medical Biochemistry, School of Medicine, The University of Balikesir, Cagis Yerleskesi, 10145 Balikesir, Turkey. ahismiogullari@gmail.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Brief Article
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World J Gastroenterol. Oct 7, 2013; 19(37): 6228-6236 Published online Oct 7, 2013. doi: 10.3748/wjg.v19.i37.6228
Isolation and biochemical analysis of vesicles from taurohyodeoxycholic acid-infused isolated perfused rat livers
Adnan Adil Hismiogullari, Sahver Ege Hismiogullari, Khalid Rahman
Adnan Adil Hismiogullari, Department of Medical Biochemistry, School of Medicine, The University of Balikesir, 10145 Balikesir, Turkey
Sahver Ege Hismiogullari, Department of Pharmacology and Toxicology, School of Veterinary Medicine, The University of Balikesir, 10145 Balikesir, Turkey
Khalid Rahman, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Adnan Adil Hismiogullari, Department of Medical Biochemistry, School of Medicine, The University of Balikesir, Cagis Yerleskesi, 10145 Balikesir, Turkey. ahismiogullari@gmail.com
Telephone: +90-26-66121455 Fax: +90-26-66121459
Received: April 19, 2013 Revised: June 12, 2013 Accepted: July 4, 2013 Published online: October 7, 2013 Processing time: 182 Days and 7.6 Hours
Core Tip
Core tip: Bile contains various constituents including cholesterol and phospholipid, mainly phosphatidylcholine with a unique fatty acid composition of 1-palmitoyl 2-linoleyl (16:0-18:2) phosphatidylcholine and 1-palmitoyl 2-oleoyl (16:0-18:1). These biliary lipids are transported in vesicles from a specific intra-hepatic pool and an increase in biliary lipid-carrying vesicles may have implications for hepatic diseases such as gallstone formation. Taurohyodeoxycholic acid (THDC) stimulates the secretion of biliary phospholipids; hence THDC-infused rat livers were subjected to ultracentrifugation in order to isolate these phospholipid-carrying vesicles. The isolation of these biliary lipid-carrying vesicles was not successful; however, vesicles enriched in PDE activity were obtained.
