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©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Aug 21, 2013; 19(31): 5159-5164
Published online Aug 21, 2013. doi: 10.3748/wjg.v19.i31.5159
Published online Aug 21, 2013. doi: 10.3748/wjg.v19.i31.5159
Effects of SAHA on proliferation and apoptosis of hepatocellular carcinoma cells and hepatitis B virus replication
Ying-Chun Wang, Xu Yang, Lan-Hua Xing, Wei-Zong Kong, Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
Author contributions: Wang YC designed research and analyzed data; Yang X performed research, analyzed data and wrote the paper; Xing LH performed research; Kong WZ performed research.
Correspondence to: Ying-Chun Wang, MD, Professor, Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Zhongshan District, Dalian 116001, Liaoning Province, China. wych_1648@126.com
Telephone: +86-411-62893717 Fax: +86-411-62893555
Received: May 20, 2013
Revised: July 4, 2013
Accepted: July 12, 2013
Published online: August 21, 2013
Processing time: 90 Days and 19 Hours
Revised: July 4, 2013
Accepted: July 12, 2013
Published online: August 21, 2013
Processing time: 90 Days and 19 Hours
Core Tip
Core tip: HepG2.2.15 cells were treated with different concentrations of suberoylanilide hydroxamic acid (SAHA). Hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) content were measured using chemiluminescence. Reverse transcription polymerase chain reaction was performed to measure hepatitis B virus (HBV) DNA in cell lysate. Results found that, the inhibitory effect of SAHA on cell proliferation was both time- and dose-dependent. After 24 h of treatment, the early cell apoptotic rate increased. After 48 h of treatment, HBsAg and HBeAg content both increased. Furthermore, HBV DNA content increased. In combination with anti-HBV drugs, SAHA may potentially be used cautiously for treatment of hepatocellular carcinoma.