Published online Oct 14, 2023. doi: 10.3748/wjg.v29.i38.5374
Peer-review started: July 27, 2023
First decision: August 8, 2023
Revised: September 16, 2023
Accepted: September 26, 2023
Article in press: September 26, 2023
Published online: October 14, 2023
Processing time: 77 Days and 7.2 Hours
Interstitial Cajal cells (ICCs) are a kind of interstitial cells existing in the gastrointestinal tract, which are related to basic electric rhythm. Many studies have shown that Interstitial Cajal-like cells (ICLCs) abnormalities are closely related to a variety of gastrointestinal dynamic disorders. ICLCs are pacemaker cells for gastrointestinal movement and are involved in the transmission of nerve impulses.
This study aims to preliminarily investigate the pathogenesis of biliary tract diseases to deepen our understanding of biliary tract dynamics and provide novel ideas for the pathogenesis and clinical treatment of related dynamic diseases.
Figure out the pathogenesis of biliary tract dynamics and related dynamic diseases such as choledocholithiasis. As well as the expression of cholecystokinin-A (CCK-A) receptors on common bile duct (CBD) ICLCs. Investigate how CCK regulates biliary tract dynamics through CBD ICLCs
Of 250-300g adult guinea pigs of either sex were used to obtain CBD tissue. Then, the levels of tyrosine kinase receptor and CCK-A receptors in CBD tissues and CBD isolated cells were quantified using the double immunofluorescence labeling technique. The enhancement effect of CCK on the movement of CBD muscle strips through CBD ICLCs was observed by muscle strip contraction test.
The guinea pig CBD ICLCs had abundant mitochondria, which were active in function and provided energy for slow-wave pacemaker cells. The expression of CCK-A receptors was confirmed on guinea pig CBD ICLCs by choledochal histology and immunofluorescence method of isolated ICLC-cultured slides.
This research demonstrates a dose-dependent contraction of smooth muscle by CCK with a concentration ranging 10-10 to 10-6 mol/L, and reduced responses of ICLC-removed CBD smooth muscle to CCK, suggesting that ICLCs not only regulate CBD smooth muscle motility by slow wave potentials caused by the known pacemaker current pathway, but also mediate CCK-induced CBD smooth muscle contraction through chemical excitation.
Investigate the distribution, expression, morphology and ultrastructure of CBD ICLCs. Also, the expression and function of CCK-A receptors on CBD ICLCs.