Case Control Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 21, 2022; 28(3): 365-380
Published online Jan 21, 2022. doi: 10.3748/wjg.v28.i3.365
Microbiome changes in the gastric mucosa and gastric juice in different histological stages of Helicobacter pylori-negative gastric cancers
Qing-Hua Sun, Jing Zhang, Yan-Yan Shi, Jing Zhang, Wei-Wei Fu, Shi-Gang Ding
Qing-Hua Sun, Jing Zhang, Jing Zhang, Wei-Wei Fu, Shi-Gang Ding, Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, China
Yan-Yan Shi, Research Center of Clinical Epidemiology, Peking University Third Hospital, Beijing 10019, China
Author contributions: Ding SG, Shi YY, and Sun QH designed the study; Sun QH analyzed the data and wrote the manuscript; Sun QH, Zhang J, Fu WW and Zhang J collected the samples and conducted the laboratory experiments; all authors approved the final version of the article.
Supported by National Natural Science Foundation of China, No. 81700496, and No. 81870386; Peking University Medicine Fund of Fostering Young Scholars’ Scientific & Technological Innovation, No. BMU2021PY002; and Key Laboratory for Helicobacter pylori Infection and Upper Gastrointestinal Diseases, Beijing Key Laboratory, No. BZ0371.
Institutional review board statement: The studies involving human participants were reviewed and approved by the Peking University Third Hospital Medical Ethics Committee (No. IRB00006761-M2017414).
Conflict-of-interest statement: No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Shi-Gang Ding, MD, Professor, Department of Gastroenterology, Peking University Third Hospital, No. 49 Huayuan North Road, Beijing 100191, China.
Received: November 26, 2021
Peer-review started: November 26, 2021
First decision: December 12, 2021
Revised: December 14, 2021
Accepted: January 11, 2022
Article in press: January 11, 2022
Published online: January 21, 2022
Research background

The gastric microbiome through the histological stages of gastric tumorigenesis remains poorly understood, especially for the Helicobacter pylori-negative gastric cancer (HPNGC).

Research motivation

To get a better knowledge of gastric microbiota and to identify microbial indicators at different histological stages of gastric tumorigenesis.

Research objectives

To identify distinct taxa in precancerous lesions and describe microbial profiles of gastric mucosa and juice for HPNGC carcinogenesis.

Research methods

We designed a clinical cohort study and utilized the 16S rRNA gene sequencing analysis.

Research results

Our study showed a change in the gastric microbial community structure along the precancerous lesions in the Helicobacter pylori-negative stages. Patients with intestinal metaplasia and dysplasia had similar gastric mucosa microbiota profiles, and their potential to be indicators for prognosis. Our findings revealed that the bacterial community of gastric juice differed from that of the gastric mucosa, and that HPNGC and its precancerous lesions have distinct bacterial taxa.

Research conclusions

Using the gastric microbiota profile, we were able to identify possible taxonomic biomarkers for HPNGC and its precancerous phases, as well as help predict prognoses for intestinal metaplasia and dysplasia.

Research perspectives

Our research revealed the core pathogenic bacteria in Helicobacter pylori-negative precancerous lesions, allowing for further investigation of the pathogenic process.