Zheng H, Wang JJ, Zhao LJ, Yang XR, Yu YL. Exosomal miR-182 regulates the effect of RECK on gallbladder cancer. World J Gastroenterol 2020; 26(9): 933-946 [PMID: 32206004 DOI: 10.3748/wjg.v26.i9.933]
Corresponding Author of This Article
Hong Zheng, MD, Chief Physician, Department of Pathology, Affiliated Hospital of Zunyi Medical University, No. 149 Dalian Road, Huichuan District, Zunyi 563003, Guizhou Province, China. zhengyao736925925@163.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Case Control Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Mar 7, 2020; 26(9): 933-946 Published online Mar 7, 2020. doi: 10.3748/wjg.v26.i9.933
Exosomal miR-182 regulates the effect of RECK on gallbladder cancer
Hong Zheng, Jin-Jing Wang, Li-Jin Zhao, Xiao-Rong Yang, Yong-Lin Yu
Hong Zheng, Jin-Jing Wang, Xiao-Rong Yang, Yong-Lin Yu, Department of Pathology, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China
Li-Jin Zhao, Department of Hepatopancreatobiliary Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China
Author contributions: Zheng H performed the majority of the experiments and analyzed the data; Wang JJ performed the molecular investigations; Zhao LJ designed and coordinated the research; Yang XR and Yu YL wrote the paper.
Institutional review board statement: This study was reviewed and approved by the Ethics Committee of Affiliated Hospital of Zunyi Medical University.
Informed consent statement: All patients in our study provided informed consent.
Conflict-of-interest statement: The authors declare no conflicts of interest.
Data sharing statement: No additional data are available.
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement-checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Hong Zheng, MD, Chief Physician, Department of Pathology, Affiliated Hospital of Zunyi Medical University, No. 149 Dalian Road, Huichuan District, Zunyi 563003, Guizhou Province, China. zhengyao736925925@163.com
Received: December 5, 2019 Peer-review started: December 5, 2019 First decision: December 30, 2019 Revised: January 8, 2020 Accepted: January 19, 2020 Article in press: January 19, 2020 Published online: March 7, 2020 Processing time: 92 Days and 0.4 Hours
ARTICLE HIGHLIGHTS
Research background
As the most common biliary malignancy, GC is an elderly-biased disease. Although extensive studies have elucidated the molecular mechanism of miR-182 and RECK in various cancers, the specific roles of exosomal miR-182 and RECK in GC remain poorly understood.
Research motivation
The expression of miR-182 and exosomal miR-182 was increased in gallbladder cancer, while RECK decreased. Targetscan7.2 predicted RECK could bind with miR-182 via 3’UTR. RECK was negatively correlated with miR-182.
Research objectives
This study was set out to explore the relationship between exosomal miR-182/RECK and metastasis of GC.
Research methods
Paired GC and adjacent normal tissues were collected from 78 patients. qPCR was employed to detect miR-182 and exosomal miR-182 expression, and Western blot was adopted to determine RECK expression. In addition, the effect of exosomal miR-182/RECK on the biological function of human GC cells were observed. Moreover, double luciferase reporter gene assay was applied to validate the targeting relationship between miR-182 and RECK.
Research results
Compared with normal gallbladder epithelial cells, miR-182 was highly expressed in GC cells, while RECK was lowly expressed. Exosomal miR-182 could be absorbed and transferred by cells. Exosomal miR-182 inhibited RECK expression and promoted migration and invasion of GC cells.
Research conclusions
Exosomal miR-182 can significantly promote the migration and invasion of GC cells by inhibiting RECK, and thus miR-182 can be used as a therapeutic target for GC.
Research perspectives
The signaling pathways implicated in miR-182/RECK should be explored in future experimental design to supplement the oncogenic network of exosomal miR-182. The clinical value of exosomal miR-182 in GC remains to be discussed.
