Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 21, 2019; 25(27): 3572-3589
Published online Jul 21, 2019. doi: 10.3748/wjg.v25.i27.3572
Mucosal healing progression after acute colitis in mice
Sandra Vidal-Lletjós, Mireille Andriamihaja, Anne Blais, Marta Grauso, Patricia Lepage, Anne-Marie Davila, Claire Gaudichon, Marion Leclerc, François Blachier, Annaïg Lan
Sandra Vidal-Lletjós, Mireille Andriamihaja, Anne Blais, Marta Grauso, Anne-Marie Davila, Claire Gaudichon, François Blachier, Annaïg Lan, UMR PNCA, AgroParisTech, INRA, Université Paris-Saclay, Paris 75005, France
Patricia Lepage, Marion Leclerc, UMR MICALIS, INRA, Université Paris-Saclay, Jouy-en-Josas 78350, France
Author contributions: Vidal-Lletjós S, Andriamihaja M, Blais A, Grauso M, and Lan A performed experiments; Vidal-Lletjós S, Lepage P, Davila AM, and Lan A analyzed the data; Lan A, Leclerc M, and Blachier F conceived and supervised the study; Vidal-Lletjós S and Lan A drafted the manuscript; All authors have read and approved the final manuscript as submitted and are accountable for all aspects of the research.
Supported by grants from the Société Française de Nutrition and the Association François Aupetit. Vidal-Lletjós S was a recipient of a PhD grant from INRA-Université Paris-Saclay (ALIAS program).
Institutional review board statement: The study was reviewed and approved by the AgroParisTech/ INRA Institutional Review Board.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the local Institutional Animal Care and Use Committee of AgroParisTech/ INRA (Comethea) and received the approval of the ministerial committee for animal experimentation (registration number: APAFIS#3987-2016012214388658), according to the European directive for the use and care of laboratory animals (2010/63/UE).
Conflict-of-interest statement: The authors have nothing to disclose.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Annaïg Lan, PhD, Associate Professor, UMR PNCA, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, Paris 75005, France. annaig.lan@agroparistech.fr
Telephone: +33-1-44087242 Fax: +33-1-44081858
Received: April 9, 2019
Peer-review started: April 10, 2019
First decision: May 9, 2019
Revised: May 27, 2019
Accepted: June 8, 2019
Article in press: June 8, 2019
Published online: July 21, 2019
Processing time: 101 Days and 21.1 Hours
ARTICLE HIGHLIGHTS
Research background

Rapid mucosal healing after an intestinal inflammatory episode is considered beneficial to diminishing the relapse risk in inflammatory bowel disease patients. However, the event progression of colon mucosal repair after a colitic flare has barely been studied.

Research motivation

A better understanding of the events associated with inflammation resolution and mucosal healing are necessary to identify potential targets for colon mucosal healing enhancement.

Research objective

To document longitudinal modifications of the colon mucosa and luminal ecosystem following an episode of chemically-induced colitis.

Research methods

Evolution of colon mucosa inflammation and healing indicators, as well the changes in colonic luminal environment, were assessed in dextran sodium sulfate-treated mice during the 3 wk after the maximal intensity of colitis. Complementary approaches such as measurement of physicochemical parameters in colonic luminal content, mucosa-adherent microbiota composition and activity, colon mucosa histo-morphological analysis, permeability tests, and expression of numerous factors involved in epithelial inflammation and/or repair were used.

Research results

Indications of epithelial repair were observed early, while inflammation was still active. However, colitis-induced luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted even though inflammation had been resolved.

Research conclusions

The longitudinal evolution study of the overlapping events that participated in epithelial repair revealed modulation factors (Il-15, Il-33, and Saa) that may prove to be potential therapeutic targets for mucosal healing enhancement.

Research perspectives

Since repairing processes were launched by mucosal inflammation, the interventional time window is an important parameter to take into account in clinical trials aiming to accelerate intestinal mucosal healing.