Colorectal Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 15, 2003; 9(6): 1231-1236
Published online Jun 15, 2003. doi: 10.3748/wjg.v9.i6.1231
Effects of KAI1/CD82 on biological behavior of human colorectal carcinoma cell line
Li Liu, De-Hua Wu, Zu-Guo Li, Guang-Zhi Yang, Yan-Qing Ding
Li Liu, Zu-Guo Li, Guang-Zhi Yang, Yan-Qing Ding, Department of Pathology, the First Military Medical University, Guangzhou 510515, Guangdong Province, China
De-Hua Wu, Department of Radiation Oncology, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation, No. 31070423, the Natural Science Foundation of Guangdong Province, No. 990385, 970335 and the Natural Science Foundation of PLA of China, No. 01MA128
Correspondence to: Dr Yang-Qing Ding, Department of Pathology, the First Military Medical University, Guangzhou 510515 Guangdong Province, China. dyq@fimmu.com
Telephone: +86-20-61642148 Fax: +86-20-61642148
Received: December 22, 2002
Revised: January 14, 2003
Accepted: February 11, 2003
Published online: June 15, 2003
Abstract

AIM: To investigate the effects of KAI1/CD82 on biological behavior of colorectal carcinoma cells.

METHODS: KAI1 cDNA was transfected into highly malignant colorectal carcinoma cell line, LoVo, which had low level of endogenous KAI1 expression, and established stable transfectant clones with high KAI1/CD82 expression. The cell-cell adhesion, cell aggregation, cell-matrix adhesion and cell invasion assay were performed to determine whether KAI1 transfectant could have an effect on proliferation, adhesion and tumor metastasis in comparison with the control transfectant cells.

RESULTS: KAI1 expression did not alter in vitro cell proliferation. But the KAI1 transfectant cells exhibited significantly increased homotypic cell-cell adhesion and cell aggregation in comparison with the control transfectant cells(P < 0.05). Furthermore, KAI1 expression significantly suppressed the cell adhesion to extracellular matrix components and in vitro cell invasion in KAI1-transfected LoVo cells. The data indicated that KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LoVo cells.

CONCLUSION: Our results suggest that KAI1 might function as a negative regulator of colorectal carcinoma metastasis.

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