Liver Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2003; 9(4): 696-700
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.696
Docetaxel inhibits SMMC-7721 human hepatocellular carcinoma cells growth and induces apoptosis
Chang-Xin Geng, Zhao-Chong Zeng, Ji-Yao Wang
Chang-Xin Geng, Ji-Yao Wang, Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Zhao-Chong Zeng, Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Prof. Ji-Yao Wang, Director of Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China. jiyao_wang@hotmail.com
Telephone: +86-21-64041990-2420 Fax: +86-21-34160980
Received: August 6, 2002
Revised: August 26, 2002
Accepted: September 3, 2002
Published online: April 15, 2003
Abstract

AIM: To investigate the in vitro anti-hepatocellular carcinoma (HCC) activity of docetaxel against SMMC-7721 HCC cells and its possible mechanism.

METHODS: The HCC cells were given different concentrations of docetaxel and their growth was measured by colony forming assay. Cell cycle and apoptosis were analyzed by flow cytometry and fluorescence microscopy (acridine orange/ethidium bromide double staining, AO/EB), as well as electronic microscopy. The SMMC-7721 HCC cell reactive oxygen species (ROS) and glutathione (GSH) were measured after given docetaxel.

RESULTS: Docetaxel inhibited the hepatocellular carcinoma cells growth in a concentration dependent manner with IC50 5 × 10-10 M. Marked cell apoptosis and G2/M phase arrest were observed after treatment with docetaxel ≥ 10-8 M. Docetaxel promoted SMMC-7721 HCC cells ROS generation and GSH deletion.

CONCLUSION: Docetaxel suppressed the growth of SMMC-7721 HCC cells in vitro by causing apoptosis and G2/M phase arrest of the human hepatoma cells, and ROS and GSH may play a key role in the inhibition of growth and induction of apoptosis.

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