Peng T, Shen HM, Liu ZM, Yan LN, Peng MH, Li LQ, Liang RX, Wei ZL, Halliwell B, Ong CN. Oxidative DNA damage in peripheral leukocytes and its association with expression and polymorphisms of hOGG1: A study of adolescents in a high risk region for hepatocellular carcinoma in China. World J Gastroenterol 2003; 9(10): 2186-2193 [PMID: 14562375 DOI: 10.3748/wjg.v9.i10.2186]
Corresponding Author of This Article
Dr. Tao Peng, Department of Hepatobiliary Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China. pengpang@hotmail.com
Article-Type of This Article
Liver Cancer
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World J Gastroenterol. Oct 15, 2003; 9(10): 2186-2193 Published online Oct 15, 2003. doi: 10.3748/wjg.v9.i10.2186
Oxidative DNA damage in peripheral leukocytes and its association with expression and polymorphisms of hOGG1: A study of adolescents in a high risk region for hepatocellular carcinoma in China
Tao Peng, Han-Ming Shen, Zhi-Ming Liu, Lu-Nan Yan, Min-Hao Peng, Le-Qun Li, Ren-Xiang Liang, Zong-Liang Wei, Barry Halliwell, Choon Nam Ong
Tao Peng, Zhi-Ming Liu, Min-Hao Peng, Le-Qun Li, Department of Hepatobiliary Sugery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China
Han-Ming Shen, Choon Nam Ong, Department of Community, Occupational and Family Medicine, National University of Singapore, Singapore
Lu-Nan Yan, Department of General Surgery, First Affiliated Hospital of West China University of Medical Sciences, Chengdu, 610041, Sichuan Zhuang Autonomous Region, China
Ren-Xiang Liang, Zong-Liang Wei, Fusui Cancer Research Institute, Fusui County, 532100, Guangxi Province, China
Barry Halliwell, Department of Biochemistry, National University of Singapore, Singapore
Author contributions: All authors contributed equally to the work.
Supported by the Guangxi Natural Sciences Grant, No.GKZ9912028 and No.GKJ0236030, Guangxi Educational Committee Grant, No. GZBH 2000-272, Guangxi Health Ministry Medicine Grant, No. Z2001087 and Singapore Science Grant, No.R-186-000-044-213
Correspondence to: Dr. Tao Peng, Department of Hepatobiliary Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China. pengpang@hotmail.com
Telephone: +86-771-5352400
Received: July 12, 2003 Revised: July 17, 2003 Accepted: July 24, 2003 Published online: October 15, 2003
Abstract
AIM: To study the oxidative DNA damage to adolescents of hepatocellular carcinoma (HCC) families in Guangxi Zhuang Autonomous Region, China.
METHODS: Peripheral leukocytes’ DNA 7,8-dihydro-8-oxoguanine (8-oxoG) and repair enzyme hOGG1 were quantified by flow-cytometry. hOGG1-Cys326Ser single nucleotide polymorphism (SNP) was distinguished by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) assay.
RESULTS: There was a positive correlation between 8-oxoG and repair enzyme hOGG1 expression (P < 0.001). HCC children (n = 21) in Fusui county had a higher level of hOGG1 (P < 0.01) and a lower level of 8-oxoG (P < 0.05) than the controls (n = 63) in Nanning city. Children in Nanning exposed to passive-smoking had a higher hOGG1 expression (P < 0.05) than the non-exposers. 8-oxoG and hOGG1 were negatively correlated with body mass index, while hOGG1 was positively correlated with age. There was a peak of 8-oxoG level nearby the 12 year point. Individuals with the hOGG1 326Ser allele had a significantly marginal higher concentration of leukocyte 8-oxoG level than hOGG1 326Cys allele.
CONCLUSION: This is the first report using flow-cytometry to simultaneously quantify both the DNA oxidative damage and its repairing enzyme hOGG1. The results provide new insights towards a better understanding of the mechanisms of oxidative stress in a population highly susceptible to hepatocarcinogenesis.
