Expression of MTLC gene in gastric carcinoma

doi:10.3748/wjg.v9.i10.2160

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Oct 15, 2003 (publication date) through May 6, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Oct 15, 2003; 9(10): 2160-2163
Published online Oct 15, 2003. doi: 10.3748/wjg.v9.i10.2160

Expression of MTLC gene in gastric carcinoma

Guang-Bin Qiu, Li-Guo Gong, Dong-Mei Hao, Zhi-Hong Zhen, Kai-Lai Sun

Guang-Bin Qiu, Li-Guo Gong, Dong-Mei Hao, Zhi-Hong Zhen, Kai-Lai Sun, Department of Medical Genetics, China Medical University, Shenyang 110001, Liaoning Province, China

Author contributions: All authors contributed equally to the work.

Supported by the National Natural Science Foundation of China, No.30171008; Funds of Educational Department, Liaoning Province, No. 20121034

Correspondence to: Kai-Lai Sun, Department of Medical Genetics, China Medical University, No.92 North 2nd Road, Heping District, Shenyang 110001, Liaoning Province, China. sunkailai@21cn.com

Telephone: +86-24-23256666-5325 Fax: +86-24-23265842

Received: March 12, 2003
Revised: May 4, 2003
Accepted: May 11, 2003
Published online: October 15, 2003

Abstract

AIM: To investigate the expression of c-myc target from laryngeal cancer cells (MTLC) gene in gastric carcinoma (GC) tissues and the effect of MTLC over-expression on gastric carcinoma cell line BGC823.

METHODS: RT-PCR was performed to determine the expression of MTLC mRNA in GC and matched control tissues. BGC823 cells were transfected with an expression vector pcDNA3.1-MTLC by liposome and screened by G418. Growth of cells expressing MTLC was observed daily by manual counting. Apoptotic cells were determined by TdT-mediated dUTP nick-end labeling (TUNEL) assay.

RESULTS: The expression of MTLC mRNAs was down-regulated in 9 (60%) of 15 cases of GC tissues. The growth rates of the BGC823 cells expressing MTLC were indistinguishable from that of control cells. A marked acceleration of apoptosis was observed in MTLC-expressing cells.

CONCLUSION: MTLC was down-regulated in the majority of GC tissues and could promote apoptosis of GC cell lines, which suggests that MTLC may play an important role in the carcinogenesis of gastric carcinoma.

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