Expression of Lewisb blood group antigen in Helicobacter pylori does not interfere with bacterial adhesion property

doi:10.3748/wjg.v9.i1.122

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Jan 15, 2003 (publication date) through Jul 22, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

H. Pylori

World J Gastroenterol. Jan 15, 2003; 9(1): 122-124
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.122

Expression of Lewis^b blood group antigen in Helicobacter pylori does not interfere with bacterial adhesion property

Peng-Yuan Zheng, Jiesong Hua, Han-Chung Ng, Khay-Guan Yeoh, Ho Bow

Peng-Yuan Zheng, Jiesong Hua, Han-Chung Ng, Ho Bow, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Republic of Singapore

Khay-Guan Yeoh, Department of Medicine, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Republic of Singapore

Author contributions: All authors contributed equally to the work.

Supported by a grant from the National University of Singapore, No. 6431

Correspondence to: Peng-Yuan Zheng, MD, PhD, Division of Gastroenterology/Nutrition, The Hospital for Sick Children, University of Toronto, 555 University Ave. Toronto, ON, Canada M5G 1X8. pengyuan.zheng@sickkids.ca

Telephone: +1-416-8137072 Fax: +1-416-8136531

Received: August 3, 2002
Revised: August 15, 2002
Accepted: August 19, 2002
Published online: January 15, 2003

Abstract

AIM: The finding that some Helicobacter pylori strains express Lewis b (Le^b) blood group antigen casts a doubt on the role of Le^b of human gastric epithelium being a receptor for H. pylori. The aim of this study was to determine if expression of Le^b in H. pylori interferes with bacterial adhesion property.

METHODS: Bacterial adhesion to immobilized Le^b on microtitre plate was performed in 63 H. pylori strains obtained from Singapore using in vitro adherence assay. Expression of Lewis blood group antigens was determined by ELISA assay.

RESULTS: Among 63 H. pylori strains, 28 expressed Le^b antigen. In vitro adhesion assay showed that 78.6% (22/28) of Le^b-positive and 74.3% (26/35) of Le^b-negative H. pylori isolates were positive for adhesion to immobilized Le^b coated on microtitre plate (P = 0.772). In addition, blocking of H. pylori Le^b by prior incubation with anti-Le^b monoclonal antibody did not alter the binding of the bacteria to solid-phase coated Le^b.

CONCLUSION: The present study suggests that expression of Le^b in H. pylori does not interfere with the bacterial adhesion property. This result supports the notion that Le^b present on human gastric epithelial cells is capable of being a receptor for H. pylori.

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