Published online Dec 15, 2002. doi: 10.3748/wjg.v8.i6.1138
Revised: February 12, 2002
Accepted: February 19, 2002
Published online: December 15, 2002
AIM: To study the correlation between expression of MMP-2, TIMP-2 protein and the ratio of MMP-2/TIMP-2 and clinical-pathological parameters of patients with gallbladder carcinoma.
METHODS: Carcinomas (n = 45) and polypoid lesions (n = 15) of the gallbladder were studied for the expression of MMP-2 and TIMP-2 protein by immunohistochemical avidin-biotin-complex method and image analysis. Clinical-pathological data of patients with gallbladder carcinoma such as histological type, grade of differentiation, level of infiltration, liver invasion and lymph node involvement, etc, were recorded.
RESULTS: There was significant difference between the average level (1.123 ± 0.108 vs 1.030 ± 0.054, P = 0.002) of MMP-2, the ratio (1.050 ± 0.013 vs 0.937 ± 0.078, P = 0.003) of MMP-2/TIMP-2 in gallbladder carcinomas and in polypoid lesions of the gallbladder. Significant difference was found between the expression of MMP-2 in early stage and advanced tumors, but there was no correlation between MMP - 2 protein expression and histological type, differentiation degree, infiltration level, lymph node involvement or liver invasion. Although no difference was observed between TIMP-2 expression and histological type or differentiation degree, significant difference was found between TIMP-2 expression and different Nevin stage, infiltration level, local lymph node involvement or liver invasion (1.168 ± 0.067 vs 1.048 ± 0.075, 1.170 ± 0.062 vs 1.039 ± 0.069, 1.039 ± 0.076 vs 1.147 ± 0.083, 1.048 ± 0.074 vs 1.103 ± 0.095, P < 0.05). MMP-2/TIMP-2 ratio did not correlate with histological type, grade of differentiation and liver invasion, but significant differences were found between MMP-2/TIMP-2 ratio and different Nevin stage, infiltration level and lymph node involvement in patients with carcinoma of gallbladder.
CONCLUSION: TIMP-2 and MMP-2/TIMP-2 ratio could reflect more accurately biological characteristic of gallbladder carcinoma and MMP-2/TIMP-2 ratio might be a new significant marker in early diagnosis, in the judgment of invasion or metastasis and the estimate of prognosis in patients with gallbladder carcinomas.