Basic Research
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 2002; 8(6): 1134-1137
Published online Dec 15, 2002. doi: 10.3748/wjg.v8.i6.1134
Effects of recombinant human growth hormone on rat septic shock with intraabdominal infection by E. coli
Ying Huang, Shu-Ren Wang, Cheng Yi, Ming-Ying Ying, Ying Lin, Mao-Hui Zhi
Ying Huang, Shu-Ren Wang, Department of Pathophysiology, Huaxi School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China
Cheng Yi, Cancer Center, Huaxi Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
Ming-Ying Ying, Ying Lin, ICU, Huaxi Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
Mao-Hui Zhi, Lab of Functional Sciences, Huaxi School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Shu-Ren Wang,Department of Pathophysiology, Huaxi School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041,Sichuan Province, China. wansuren@mail.sc.cninfo.net
Telephone: +86-28-85501268
Received: June 23, 2002
Revised: August 3, 2002
Accepted: August 16, 2002
Published online: December 15, 2002
Abstract

AIM: To investigate the therapeutic effects of recombinant human growth hormone (rhGH) on rat septic shock with intraabdominal infection by E. coli and its possible mechanism.

METHODS: 76 SD rats were divided into 3 groups randomly: control group (group C, n = 16) without any special treatment, septic shock group (group S, n = 30) received bolus injection of E.coli (1 × 1010 cfu·L-1,15 mL·kg-1, ip), treated group (group T, n = 30) received bolus injection of E.coli, and then followed by rhGH injection (2.25 U·kg-1·d-1, im). Group S and group T were further divided into 1d and 3d subgroups, respectively (n = 15 each). Mean arterial pressure (MAP), levels of plasma TNFα and endotoxin and leukocyte count were determined on 1st day and 3rd day after E.coli injection. Another 39 SD rats were divided into groups C, S and T (n = 13 each) just for observing survival rate within 1 week.

RESULTS: (1) On 1st and 3rd day, MAP in group S decreased markedly, and MAP on 1st day lowered more than that of 3rd day (P < 0.01), while MAP in group T just decreased slightly. The survival rate within 1 week was much higher in group T (84.6%) than in group S (46.2%) (P < 0.01). (2)On 1st day, plasma TNFα and endotoxin elevated significantly in group S and group T (P < 0.05), and endotoxin in group S had more increase (P < 0.01). On 3rd day, TNFα in group S returned to the level of group C (P > 0.05),while TNFα in group T went down below the level of group C(P < 0.01). On 3rd day, endotoxin in group S declined, but was still higher than that of group C (P < 0.01), endotoxin in group T returned to the level of group C (P > 0.05). (3) On 1st day, neutrophil ratio in total leukocyte count in both group S and group T increased significantly (P < 0.05 vs group C).

CONCLUSION: rhGH showed beneficial effects on rat septic shock. The possible mechanisms may involve the attenuation of bacteria/endotoxin translocation and decreased systemic endotoxin level; inhibition of the production and release of TNFα; improved circulatory function; improved systemic host defenses and maintenance of intestinal mucosa barrier.

Keywords: $[Keywords]