Viral Hepatitis
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 2002; 8(6): 1081-1087
Published online Dec 15, 2002. doi: 10.3748/wjg.v8.i6.1081
Prevalence of hepatitis G virus infection and homology of different viral strains in Southern China
Gang Li, Hui-Hui Ma, Geroge KK Lau, Yin-Kit Leung, Chun-Lan Yao, Yu-Tian Chong, Wen-Hui Tang, Ji-Lu Yao
Gang Li, Hui-Hui Ma, Chun-Lan Yao, Yu-Tian Chong, Ji-Lu Yao, Department of Infectious Diseases, the Third Affiliated Hospital, Zhongshan University, Guangzhou 510630, Guangdong Province, China
Geroge KK Lau, Division of Gastroenterology and Hepatology, University Department of Medicine, Queen Mary Hospital, Hong Kong Special Administrative Region, China
Yin-Kit Leung, Viral Hepatitis Research Center of Guangdong-Hong Kong, Hong Kong Division, Hong Kong Special Administrative Region, China
Wen-Hui Tang, Department of Infectious Diseases, the First Affiliated Hospital, Kunming Medical College, Yunnan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, NO. 39600130 and the grant from the Department of Health of Guangdong Province.
Correspondence to: Dr. Gang Li, Department of Infectious Diseases, the Third Affiliated Hospital, Zhongshan University, Gangding, Shipai, Guangzhou 510630, Guangdong Province, China.
Telephone: +86-20-85516867-2019 Fax: +86-20-87544614
Received: April 10, 2002
Revised: May 12, 2002
Accepted: May 18, 2002
Published online: December 15, 2002

AIM: To investigate the prevalence of hepatitis G virus (HGV) infection and to analyse the homology of different HGV strains in Southern China.

METHODS: A total of 1993 sera from different groups in Guangdong, Hong Kong, and Yunnan were detected by reverse transcription polymerase chain reaction (RT-PCR). The nucleotide sequences of 5’untranslated region (5’UTR) derived from 20 strains and NS5 region from 3 strains were determined.

RESULTS: The positive rate of HGV RNA was 0.89% in community population, 2.57% in blood donors, 17.86% in intravenous drug abusers, 14.13% in patients with hemodialysis, 13.66% in those with hepatocellular carcinoma, 25.30% in non A-E hepatitis, 7.22% in hepatitis B, 12.73% in hepatitis C, 41.67% in patients received bone marrow transplantation, respectively. The homology was 90.40%-100% in 5’UTR among different strains, while that of NS5 region was 93.3%-94% in nucleotide sequence, and 97%-99.2% in amino acid sequence.

CONCLUSION: These results showed that there was a high incidence of HGV infection in patients from Southern China, being treated for bone marrow transplantation, hepatocellular carcinoma and those on haemodialysis. Furthermore, there was also a high frequency of co-infection of HGV with HBV, HCV, non A-E viral hepatitis and that among intravenous drug abusers. The study also showed that sequence variation in different strains was associated with geographical factors but there was no significant difference in 5’UTR in circulating viruses between different patient groups. Finally, by sequential analysis of viral species present in individual patients over a three months period there was no evidence of sequence variation in the 5' UTR.

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