Basic Research
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 15, 2002; 8(3): 555-557
Published online Jun 15, 2002. doi: 10.3748/wjg.v8.i3.555
The effects of anisodamine and dobutamine on gut mucosal blood flow during gut ischemia/reperfusion
Sen Hu, Zhi-Yong Sheng
Sen Hu, Zhi-Yong Sheng, Burns Institute, 304th Hospital of PLA, Beijing 100037, China
Author contributions: All authors contributed equally to the work.
Supported by the Tenth Five-Year Key Project of PLA, No. 01L081
Correspondence to: Dr Sen Hu, Burns Institute, 304th Hospital of PLA, 51 Fu Cheng Road, Beijing 100037, China
Telephone: +86-10-66867397 Fax:+86-10-68429998
Received: January 26, 2002
Revised: February 3, 2002
Accepted: February 20, 2002
Published online: June 15, 2002
Abstract

AIM: To determine if anisodamine is able to augment mucosal perfusion during gut I/R ischemia-reperfusion.

METHODS: A jejunal sac was formed in Sprague Dawley rat. A Laser Doppler probe and a tonometer were inserted into the sac which was filled with saline. The superior mesenteric artery was occluded (SMAO) for 60 minutes followed by 90 minutes of reperfusion. At the end of 60 minutes of SMAO, either 0.2 mg/kg of anisodmine or dobutamine was injected into the jejunal sac. Laser Doppler mucosal blood flow and regional PCO2 (PrCO2) measurements were made.

RESULTS: Mucosal blood flow was significantly increased at 30, 60 and 90 minutes of reperfusion (R30, R60, R90) when intraluminal anisodamine or dobutamine was present compared to intraluminal saline only (44 ± 3.3% or 48 ± 4.1% vs 37 ± 2.6% at R30, 57 ± 5.0% or 56 ± 4.7% vs 45 ± 2.7% at R60, 64 ± 3.3% or 56 ± 4.2% vs 48 ± 3.4% at R90, respectively P < 0.05). Blood flow changes were also reflected by lowering of jejunal PrCO2 measurements after intraluminal anisodamine or dobutamine compared with that of the saline controls (41 ± 3.1 mmHg or 44 ± 3.0 mmHg vs 49 ± 3.7 mmHg at R30, 38 ± 3.7 mmHg or 40 ± 2.1 mmHg vs 47 ± 3.8 mmHg at R60, 34 ± 2.1 mmHg or 39 ± 3.0 mmHg vs 46 ± 3.4 mmHg at R90, respectively, P < 0.05). Most interesting finding was that there were significantly higher mucosal blood flow and lower jejunal PrCO2 in anisodamine group than those in dobutamine group at 90 minutes of reperfusion (64 ± 3.3% vs 56 ± 4.2% for blood flow or 34 ± 2.1 mmHg vs 39 ± 3.0 mmHg for PrCO2, respectively, P < 0.05), suggesting that anisodamine had a more lasting effect on mucosal perfusion than dobutamine.

CONCLUSION: Intraluminal anisodamine and dobutamine can augment mucosal blood flow during gut I/R and alleviate mucosal acidosis. The results provided benificial effects on the treatment of splanchnic hypoperfusion following traumatic or burn shock.

Keywords: $[Keywords]