Original Research
Copyright ©The Author(s) 2001. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2001; 7(4): 515-521
Published online Aug 15, 2001. doi: 10.3748/wjg.v7.i4.515
Expression, deleton and mutation of p16 gene in human gastric cancer
Xiu-Sheng He, Qi Su, Zhu-Chu Chen, Xiu-Tao He, Zhi-Feng Long, Hui Ling, Liang-Run Zhang
Xiu-Sheng He, Qi Su, Zhi-Feng Long, Hui Ling, Liang-Run Zhang, Oncology Institute, Nanhua University, Hengyang 421001, Hunan Province, China
Zhu-Chu Chen, Oncology Institute, Center South University, Changsha 410078, Hunan Province, China
Xiu-Tao He, Department of Gastroenterology, First People’s Hospital of Changde City, Changde 415003, Hunan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the grant from the Teaching Committee of Hunan Province, No. 97B095, and the “8th 5 year Plan”of Health Department of Hunan Province, No. 9301
Correspondence to: Dr. Xiu-Sheng He, The Oncology Institute of Center South University, Changsha 410078, Hunan Province, China. hxs59@hotmail.com
Telephone: 0086-731-4805447 Fax: 0086-731-4485482
Received: February 20, 2001
Revised: May 13, 2001
Accepted: May 20, 2001
Published online: August 15, 2001
Abstract

AIM: To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis, depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma.

METHODS: The expression of P16 protein was examined by streptavidin-peroxidase conjugated method (S-P); the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction (PCR) and polymerase chain reaction single strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma.

RESULTS: Expression of P16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/50) of the dysplastic gastric mucosa and in 47.54% (58/122) of the gastric carcinoma. The positive rate of P16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P < 0.05). The positive rate of P16 protein expression in mucoid carcinoma 10.00% (1/10) was significantly lower than that in poorly differentiated carcinoma 51.22% (21/41), undifferentiated carcinoma 57.69% (15/26) and signet ring cell carcinoma 62.50% (10/16) (P < 0.05). The positive rate of p16 protein in 30 cases paired primary and lymph node metastatic gastric carcinoma: There was 46.67% (14/30) in primary gastric carcinoma, 16.67% (5/30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P < 0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16 gene in exon 2 in the 25 primary gastric carcinomas.

CONCLUSIONS: The expression loss of P16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph merastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis.

Keywords: gastric carcinoma, dysplasis p16/MTS1/CDK4I/CDKN2 gene, mutation deletion, expression, stomach neoplasms, genetics genes