Original Articles
Copyright ©The Author(s) 1999. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 1999; 5(5): 404-407
Published online Oct 15, 1999. doi: 10.3748/wjg.v5.i5.404
Inhibitory activity of polysaccharide extracts from three kinds of edible fungi on proliferation of human hepatoma SMMC-7721 cell and mouse implanted S180 tumor
Shi-Ming Jiang, Zheng-Ming Xiao, Zhao-Hui Xu
Shi-Ming Jiang, Zheng-Ming Xiao, Zhao-Hui Xu, Biology Department, the Provincial Key Laboratory of Animal Stress, Shandong Normal University, Jinan 250014, Shandong Province, China
Shi-Ming Jiang, male, born on 1961-11-26 in Zhaoyuan City, Shandong Province, graduated from Shandong Normal University as a postgraduate in 1985, now associate professor of cell biology, an M.Ph.D. candidate of the Heidelberg University, Germany, having more than 30 papers and one book published.
Author contributions: All authors contributed equally to the work.
Supported by a grant from the Science and Technology Committee of Shandong Province, No.95071910.
Correspondence to: Dr. Shi-Ming Jiang, Biology Department, Shan dong Normal University, Jinan 250014, Shandong Province, China. S.Jiang@DKFZ-Heidelberg.de
Received: April 8, 1999
Revised: August 20, 1999
Accepted: September 24, 1999
Published online: October 15, 1999

AIM: To determine the activities of polysaccharide extracts from Flammulina velutipes (Curt. ex Fr.) Sing (FV), Lentinus edodes (LE) and Agaricus bisporus-Sing (AB) on the proliferation of human hepatoma SMMC-7721 cells in vitro and on mouse implanted S-180 tumors in vivo.

METHODS: The polysaccharide extracts were isolated from the fruit bodies of FV, LE and AB by the methods of hot-water extraction, Sevag’s removal of proteins, ethanol precipitation, trypsin digestion and ethanol fractional precipitation. Human hepatoma SMMC-7721 cells were treated with 50 mg/L polysaccharide extracts, and the mitosis index, mitochondria activity and cell proliferation were detected at different times in both control and experimental groups. The mice with S-180 implanted tumors were injected with the polysaccharide extracts at 24 mg/kg body weight for 9 d and the tumor weight was measured on the 15th day.

RESULTS: The mitosis index of hepatoma cells in vitro could be significantly decreased by treatment with the polysaccharide extracts from the three kinds of edible fungi (P < 0.005). The cell numbers and mitochondria activity of SMMC-7721 cells treated with polysaccharide extracts were lower than those in control groups (P < 0.005). The inhibition rates of polysaccharide extracts against implanted S-180 tumors in mice were 52.8%, 56.6% and 51.9% respectively compared with that in control groups.

CONCLUSION: The polysaccharide extracts from the three kinds o f edible fungi could inhibit not only the cultured malignant cells in vitro but also implanted S-180 tumor in vivo.

Keywords: polysaccharide, edible fungi, liver neoplasm, carcinoma, hepatocellular, SMMC-7721, tumor cell, cultured, implanted tumor, S-180, cell proliferation