Two novel gastric cancer-associated genes identified by differential display

doi:10.3748/wjg.v4.i4.334

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 15, 1998; 4(4): 334-336
Published online Aug 15, 1998. doi: 10.3748/wjg.v4.i4.334

Two novel gastric cancer-associated genes identified by differential display

Han You, Bing Xiao, Da-Xiang Cui, Yong-Quan Shi, Dai-Ming Fan

Han You, Bing Xiao, Da-Xiang Cui, Yong-Quan Shi, Dai-Ming Fan, Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Han You, female, born on 1974-04-12 in Chengdu City, postgraduate studying in Fourth Military Medical University, majoring cloning and analysing novel gastric cancer-associated genes.

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Han You, Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Telephone: +86-29-2539041

Received: June 6, 1998
Revised: July 16, 1998
Accepted: July 21, 1998
Published online: August 15, 1998

Abstract

AIM: To clone novel gastric cancer-associated genes and investigate their roles in gastric cancer occurrence.

METHODS: A method called differential display was used which allows the identification of differentially expressed genes by using PAGE to display PCR-amplified cDNA fragments between gastric cancer cells and normal gastric mucosa cells. These fragments were cloned into plasmid vector pUC18. Homology analysis was made after sequencing these fragments.

RESULTS: Two novel genes were identified compared with sequences from GenBank. One was registered with the AD number AF 051783. In situ hybridization showed that these two novel genes expressed specifically in gastric cancer tissues.

CONCLUSION: The two novel genes obtained by differential display were confirmed to be gastric cancer-associated genes using in situ hybridization.

Keywords: stomach neoplasms; gene clone; nucleotides sequence analysis; in situ hybridization; polymerase chain reaction; RNA, messenger