Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 1998; 4(4): 314-316
Published online Aug 15, 1998. doi: 10.3748/wjg.v4.i4.314
Phase tissue intercellular adhesion molecule-1 expression in nude mice human liver cancer metastasis model
Jing-Jing Sun, Xin-Da Zhou, Yin-Kun Liu, Ge Zhou
Jing-Jing Sun, Xin-Da Zhou, Yin-Kun Liu, Ge Zhou, Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China
Jing-Jing Sun, female, born on 1966-01-08 in Hegang, Heilongjiang Province, graduated from Harbin Medical College in 1986, worked in the First Hospital of Harbin Medical College as attending doctor of general surgery, now is studying in Shanghai Medical University as a Ph. D. student, majoring liver surgery, having 16 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Prof. Xin-Da Zhou, Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China
Telephone: +86-21-64041990 ext 2736 Fax: +86-21-64037181
Received: July 6, 1998
Revised: July 22, 1998
Accepted: August 4, 1998
Published online: August 15, 1998
Abstract

AIM: To study the phase cancer tissue intercellular adhesion molecule-1 (ICAM-1) expression of human cancer metastasis model in nude mice, and to analyze the relationship between ICAM-1 expression and the metastasis and recurrence of hepatocellular cancinoma (HCC).

METHODS: HCC tissues from liver cancer metastasis model in nude mice (LCI-D20) was orthotopically implanted, and ICAM-1 expression in HCC tissues at different growing time were detected by immunodot blot. Tumor size, intrahepatic and extrahepatic metastasis foci were observed by naked eyes and under light microscope.

RESULTS: ICAM-1 was positively correlated to the tumor growing time (r = 0.88, P < 0.01) and tumor size r = 0.5, P < 0.05). It was higher in metastatic HCC than in nonmetastatic HCC (8.24 ± 0.95 vs 3.03 ± 0.51, P < 0.01). ICAM-1 content in cancer tissues increased suddenly after metastasis occurred and then maintained in a high level. ICAM-1 was also higher in multimetastasis group than in monometastasis group (10.05 ± 1.17 vs 5.48 ± 0.49, P < 0.05).

CONCLUSION: Tissue ICAM-1 could predict not only the metastasis of human liver cancer metastasis model in nude mice early and sensitively, but also the metastasis degree. So tissue ICAM-1 may be a potential index indicating the status of metastasis of HCC patients.

Keywords: liver neoplasms; carcinoma, hepatocellular; neoplasm metastasis; intercellular adhesion molecule-1; disease models, animal