Mi L, Zhang K, Ma JX, Yao JF, Tong YL, Bao ZJ. Hollow cerium nanoparticles synthesized by one-step method for multienzyme activity to reduce colitis in mice. World J Gastroenterol 2025; 31(5): 98732 [DOI: 10.3748/wjg.v31.i5.98732]
Corresponding Author of This Article
Zhi-Jun Bao, MD, Professor, Department of Gastroenterology, Huadong Hospital, Fudan University, No. 221 West Yan’an Road, Shanghai 200040, China. zhijunbao@fudan.edu.cn
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Feb 7, 2025; 31(5): 98732 Published online Feb 7, 2025. doi: 10.3748/wjg.v31.i5.98732
Hollow cerium nanoparticles synthesized by one-step method for multienzyme activity to reduce colitis in mice
Lin Mi, Kai Zhang, Jian-Xia Ma, Jian-Feng Yao, Yi-Li Tong, Zhi-Jun Bao
Lin Mi, Kai Zhang, Yi-Li Tong, Department of General Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China
Jian-Xia Ma, Jian-Feng Yao, Zhi-Jun Bao, Department of Gastroenterology, Huadong Hospital, Fudan University, Shanghai 200040, China
Author contributions: Mi L designed the research and wrote the paper; Mi L, Zhang K, and Tong YL performed the majority of the experiments; Mi L, Ma JX, Yao JF, and Bao ZJ analysed the data.
Institutional animal care and use committee statement: This study has received ethical approval for animal experiments, No. 2022JS Huadong Hospital-081.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: All relevant data are within the manuscript and its additional files.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Zhi-Jun Bao, MD, Professor, Department of Gastroenterology, Huadong Hospital, Fudan University, No. 221 West Yan’an Road, Shanghai 200040, China. zhijunbao@fudan.edu.cn
Received: July 4, 2024 Revised: October 8, 2024 Accepted: December 4, 2024 Published online: February 7, 2025 Processing time: 179 Days and 5.4 Hours
Abstract
BACKGROUND
Inflammatory bowel disease (IBD) is a common chronic intestinal inflammatory disease. High oxidative stress is a treatment target for IBD. Cerium oxide (CeO2) nanomaterials as nanozymes with antioxidant activity are potential drugs for the treatment of colitis.
AIM
To synthesize hollow cerium (H-CeO2) nanoparticles by one-step method and to validate the therapeutic efficacy of H-CeO2 in IBD.
METHODS
H-CeO2 was synthesized by one-step method and examined its characterization and nanoenzymatic activity. Subsequently, we constructed dextran sulfate sodium (DSS)-induced colitis in mice to observe the effects of H-CeO2 on colonic inflammation. The effects of H-CeO2 on colon inflammation and reactive oxygen species (ROS) levels in IBD mice were detected by hematoxylin and eosin staining and dichlorofluorescein diacetate staining, respectively. Finally, the biological safety of H-CeO2 on mice was evaluated by hematoxylin and eosin staining, blood routine, and blood biochemistry.
RESULTS
H-CeO2 nanoparticles prepared by the one-step method were uniform, monodisperse and hollow. H-CeO2 had a good ability to scavenge ROS, ∙OH and ∙OOH. H-CeO2 reduced DSS-induced decreases in body weight and colon length, colonic epithelial damage, inflammatory infiltration, and ROS accumulation. H-CeO2 administration reduced the disease activity index of DSS-induced animals from about 8 to 5. H-CeO2 had no significant effect on body weight, total platelet count, hemoglobin, white blood cell, and red blood cell counts in healthy mice. No significant damage to major organs was observed in healthy mice following H-CeO2 administration.
CONCLUSION
The one-step synthesis of H-CeO2 nanomaterials had good antioxidant activity, biosafety, and inhibited development of DSS-induced IBD in mice by scavenging ROS.
Core Tip: Inflammatory bowel disease (IBD) is a common chronic intestinal inflammatory disease. High oxidative stress is a treatment target for IBD. Cerium oxide nanomaterials as nanozymes with antioxidant activity are potential drugs for the treatment of colitis. To synthesize hollow cerium (H-CeO2) nanoparticles by one-step method and to study the physicochemical properties, biosafety, and therapeutic efficacy of H-CeO2 in IBD. In this study, we found that a one-step synthesis of H-CeO2 nanomaterials has good antioxidant activity, biosafety, and can inhibit the development of dextran sulphate sodium-induced IBD in mice by scavenging reactive oxygen species.
