Gao J, Cao GW, Qi ZT, Qiu XF, Wu ZD, Du P, Yang WG, Cui L. Construction of retroviral vector carrying HSV-tk gene under control of human AFP enhancer core sequence and human pgk promotor. World J Gastroenterol 1997; 3(1): 9-11 [PMID: 27006574 DOI: 10.3748/wjg.v3.i1.9]
Corresponding Author of This Article
Dr. Jun Gao, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China
Article-Type of This Article
Original Research
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Mar 15, 1997; 3(1): 9-11 Published online Mar 15, 1997. doi: 10.3748/wjg.v3.i1.9
Construction of retroviral vector carrying HSV-tk gene under control of human AFP enhancer core sequence and human pgk promotor
Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Long Cui
Jun Gao, Guang-Wen Cao, Zhong-Tian Qi, Xiao-Fang Qiu, Zhong-Di Wu, Ping Du, Wen-Guo Yang, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China
Long Cui, Department of General Surgery, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China
Jun Gao, male, born on January 7, 1967 in Xianyang City of Shaanxi Pro vince, and graduated from the Fourth Military Medical University, is engaged in cancer gene therapy, having 3 papers published
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Jun Gao, Department of Microbiology, Faculty of Basic Medical Sciences, the Second Military Medical University, Shanghai 200433, China
Telephone: +86-21-65347018
Received: August 8, 1996 Revised: September 30, 1996 Accepted: January 1, 1997 Published online: March 15, 1997
Abstract
AIM: To construct retroviral vector bringing HSV-tk gene under control of human AFP enhancer core sequence and human pgk promoter.
METHODS: Internal SV40 promoter was deleted by SalI from retroviral vector pMNSM to construct pMNM. HSV-tk gene driven by pgk promoter was released by BamH I from an eukaryotic expression vector pBPGK-tk, and inserted into polylinker site of pMNM to construct pMNP-tk retroviral vector. Human α-fetoprotein gene enhancer core sequence was released by EcoR I from pGEM. 7Z-AFPe plasmid was inserted into the immediate upstream of pgk promoter of pMNP-tk vector. Construction of hepatoma specific retroviral vector pMNAP-tk was completed.
RESULTS: The structure of pMNP-tk and pMNAP-tk vector was confirmed by restriction analysis.
CONCLUSION: The vector is of great significance for hepatoma specific prodrug transformation gene therapy.
