Analysis of lactate dehydrogenase activities and isoenzyme patterns in colorectal cancer tissues

doi:10.3748/wjg.v3.i1.41

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 15, 1997; 3(1): 41-42
Published online Mar 15, 1997. doi: 10.3748/wjg.v3.i1.41

Analysis of lactate dehydrogenase activities and isoenzyme patterns in colorectal cancer tissues

Chun-Hua Zhao, Chun-Ying Jiang, Yu-Yi Zhang, Xian-Xi Liu, Dao-Chun Luo, Xiao-Ting Zhang, Yu-Qin Lin

Chun-Hua Zhao, Yu-Yi Zhang, Xian-Xi Liu, Dao-Chun Luo, Yu-Qin Lin, The Department of Biochemistry, Shandong Medical University, Jinan 250012, Shandong Province, China

Chun-Ying Jiang, Xiao-Ting Zhang, Shandong Traditional Hospital, Jinan 250012, Shandong Province, China

Chun-Hua Zhao, with 8 published papers, Lecturer of Biochemistry

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Chun-Hua Zhao, The Department of Biochemistry, Shandong Medical University, Jinan 250012, Shandong Province, China

Telephone: +86-531-2952424-693

Received: July 1, 1996
Revised: September 1, 1996
Accepted: January 1, 1997
Published online: March 15, 1997

Abstract

AIM: To investigate the relationship between lactate dehydrogenase (LDH) activity or LDH isoenzyme patterns and the pathogenesis of colorectal cancer.

METHODS: Activities of tissue LDH and LDH isoenzyme patterns in 16 patients with colorectal cancer were assayed using spectrophotometric procedures and agarose gel electrophoresis, respectively.

RESULTS: The total and specific activities of LDH were significantly higher in colorectal cancer tissues than those in adjacent noncancerous tissues (P < 0.001). The LDH isoenzyme pattern was also different from that in the control. The percentage of LDH₅ doubled and the ratio of LDH₄ + LDH₅/LDH₁ + LDH₂ was 3.6 ± 1.4 in cancer tissue, significantly greater than in the control.

CONCLUSIONS: The increased LDH activity in colorectal cancer tissues resulted mainly from the increased LDH₅, suggesting that the alteration of LDH activity and isoenzyme patterns were related to the pathogenesis of colorectal cancer.

Keywords: Colonic neoplasms, Rectal neoplasms, Lactate dehydrogenase, Lactate dehydrogenase isoenzymes