Published online Oct 14, 2019. doi: 10.3748/wjg.v25.i38.5789
Peer-review started: July 29, 2019
First decision: August 17, 2019
Revised: August 30, 2019
Accepted: September 13, 2019
Article in press: September 13, 2019
Published online: October 14, 2019
Processing time: 77 Days and 1.7 Hours
Hepatocellular carcinoma (HCC) has become a great threat for people’s health. Many long noncoding RNAs are involved in the pathogenesis of HCC. SNHG15, as a tissue specific long noncoding RNAs, has been studied in many human cancers, except HCC.
To explore the regulatory mechanism of SNHG15 in HCC.
In the present research, 101 HCC patient samples, two HCC cell lines and one normal liver cell line were used. RT-qPCR and Western blot analysis were applied to detect SNHG15, miR-490-3p and histone deacetylase 2 (HDAC2) expression. The regulatory mechanism of SNHG15 was investigated using CCK-8, Transwell and luciferase reporter assays.
Our research showed that up-regulation of SNHG15 was found in HCC and was related to aggressive behaviors in HCC patients. Moreover, knockdown of SNHG15 restrained HCC cell proliferation, migration and invasion. In addition, SNHG15 served as a molecular sponge for miR-490-3p. Further, miR-490-3p directly targets HDAC2. HDAC2 was involved in HCC progression by interacting with the SNHG15/miR-490-3p axis.
In conclusion, long noncoding RNA SNHG15 promotes HCC progression by mediating the miR-490-3p/HDAC2 axis in HCC.
Core tip: Long noncoding RNA (lncRNA)-SNHG15 is up-regulated in hepatocellular carcinoma (HCC) tissue and cell lines. HCC patients with up-regulated lncRNA-SNHG15 level were more likely to have larger tumor sizes and advanced clinical stage. lncRNA-SNHG15 promotes cell proliferation, migration and invasion in HCC cell lines. The miR-490-3p/histone deacetylase 2 axis was determined to be the target regulated by lncRNA-SNHG15 in HCC, and the regulatory mechanism of lncRNA-SNHG15 has been preliminarily illuminated.