Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 14, 2019; 25(38): 5789-5799
Published online Oct 14, 2019. doi: 10.3748/wjg.v25.i38.5789
lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis
Wei Dai, Jia-Liang Dai, Mao-Hua Tang, Mu-Shi Ye, Shuo Fang
Wei Dai, Jia-Liang Dai, Department of Hepatobiliary Surgery, the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, Guangdong Province, China
Mao-Hua Tang, Department of Infectious Disease, the Second Affiliated Hospital of Guangdong Medical University, Zhanjiang 524003, Guangdong Province, China
Mu-Shi Ye, Department of Surgery, the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, Guangdong Province, China
Shuo Fang, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, Guangdong Province, China
Shuo Fang, Li KaShing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Author contributions: Dai W and Dai JL performed the majority of experiments and analyzed the data; Ye MS performed the molecular investigations; Tang MH designed and coordinated the research; Fang S wrote the paper.
Institutional review board statement: This study was reviewed and approved by the Affiliated Hospital of Guangdong Medical University Ethics Committee.
Informed consent statement: All patients in our study provided informed consent.
Conflict-of-interest statement: The authors declare no conflict of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Shuo Fang, PhD, Attending Doctor, The Seventh Affiliated Hospital, Sun Yat-sen University, No. 628, Zhenyuan Road, Xinhu Street, Guangming New District, Shenzhen 518107, Guangdong Province, China. shuofangzhongshan@163.com
Telephone: +86-852-65923702 Fax: +86-852-65923702
Received: July 29, 2019
Peer-review started: July 29, 2019
First decision: August 17, 2019
Revised: August 30, 2019
Accepted: September 13, 2019
Article in press: September 13, 2019
Published online: October 14, 2019
Abstract
BACKGROUND

Hepatocellular carcinoma (HCC) has become a great threat for people’s health. Many long noncoding RNAs are involved in the pathogenesis of HCC. SNHG15, as a tissue specific long noncoding RNAs, has been studied in many human cancers, except HCC.

AIM

To explore the regulatory mechanism of SNHG15 in HCC.

METHODS

In the present research, 101 HCC patient samples, two HCC cell lines and one normal liver cell line were used. RT-qPCR and Western blot analysis were applied to detect SNHG15, miR-490-3p and histone deacetylase 2 (HDAC2) expression. The regulatory mechanism of SNHG15 was investigated using CCK-8, Transwell and luciferase reporter assays.

RESULTS

Our research showed that up-regulation of SNHG15 was found in HCC and was related to aggressive behaviors in HCC patients. Moreover, knockdown of SNHG15 restrained HCC cell proliferation, migration and invasion. In addition, SNHG15 served as a molecular sponge for miR-490-3p. Further, miR-490-3p directly targets HDAC2. HDAC2 was involved in HCC progression by interacting with the SNHG15/miR-490-3p axis.

CONCLUSION

In conclusion, long noncoding RNA SNHG15 promotes HCC progression by mediating the miR-490-3p/HDAC2 axis in HCC.

Keywords: SNHG15, miR-490-3p, Hepatocellular carcinoma, Histone deacetylase 2, Pathogenesis, Regulatory mechanism

Core tip: Long noncoding RNA (lncRNA)-SNHG15 is up-regulated in hepatocellular carcinoma (HCC) tissue and cell lines. HCC patients with up-regulated lncRNA-SNHG15 level were more likely to have larger tumor sizes and advanced clinical stage. lncRNA-SNHG15 promotes cell proliferation, migration and invasion in HCC cell lines. The miR-490-3p/histone deacetylase 2 axis was determined to be the target regulated by lncRNA-SNHG15 in HCC, and the regulatory mechanism of lncRNA-SNHG15 has been preliminarily illuminated.