Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 14, 2017; 23(38): 7009-7015
Published online Oct 14, 2017. doi: 10.3748/wjg.v23.i38.7009
Expression of annexin II in gastric carcinoma and its role in gastric cancer metastasis
Feng Han, Shikha Shrestha, Hua Huang, Huang-Yong Lv, Chuan Nie, Ling Lin, Ming-Liang Lu
Feng Han, Huang-Yong Lv, Chuan Nie, Ling Lin, Department of Gastroenterology, the PLA No. 59 Hospital, Kaiyuan 661600, Yunnan Province, China
Feng Han, Shikha Shrestha, Hua Huang, Ming-Liang Lu, Department of Gastroenterology, The Second Affiliated Hospital, Kunming Medical University, Kunming 650101, Yunnan Province, China
Author contributions: Lu ML and Huang H designed the research, and controlled the structure and quality of the paper; Han F and Shrestha S proposed the study and collected and analyzed the data, and wrote the first draft; Lv Hy, Nie C and Lin L enriched and improved the discussion section.
Institutional review board statement: The medical records and research data of the subjects are kept confidential. When the results are published, Information related to the subject will not be disclosed. The research program meets the medical ethics. The research topic is ethical.
Conflict-of-interest statement: All the authors have no conflicts of interests to declare.
Data sharing statement: No additional unpublished data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Ming-liang Lu, MD, Associate Professor, Department of Gastroenterology, the Second Affiliated Hospital, Kunming Medical University, Kunming 650101, Yunnan Province, China. lml19910@163.com
Telephone: +86-13187818116 Fax: +86-871-65361017
Received: May 12, 2017
Peer-review started: May 16, 2017
First decision: June 22, 2017
Revised: July 30, 2017
Accepted: August 25, 2017
Article in press: August 25, 2017
Published online: October 14, 2017
Processing time: 156 Days and 9.2 Hours
Abstract
AIM

To investigate the expression of annexin II in gastric carcinoma and its role in the metastasis of gastric cancer.

METHODS

The expression of annexin II in 51 cases of gastric carcinoma and 24 cases of adjacent tissues was detected by immunohistochemistry. The relationship between annexin II and clinical features of gastric cancer was analyzed. Annexin II specific siRNA was used to inhibit the expression of annexin II in gastric cancer HGC-27 cells, and the effects of annexin II on the migration and secretion of matrix metalloproteinases (MMPs) were observed.

RESULTS

The positive rate of annexin II protein was 82.4% in gastric cancer tissues and 37.5% in adjacent tissues. There was significant difference between the two groups (P < 0.01); and the positive expression of annexin II was not related to the sex and age of the patients (P > 0.05). The expression of annexin II protein was correlated with tumor size, histological differentiation, TNM stage, Lymph node metastasis and other clinical features were significantly correlated, the difference was statistically significant (P < 0.05). Inhibition of annexin II expression, gastric cancer HGC-27 cells migration and secretion of MMPs were significantly decreased, the difference was statistically significant (P < 0.05).

CONCLUSION

Annexin II is highly expressed in gastric cancer tissues, annexin II protein expression is related to tumor size, histological differentiation, TNM staging, lymph node metastasis and other clinical features were significantly correlated. Annexin II high expression can promote the invasion and metastasis of gastric cancer.

Keywords: Gastric cancer; Annexin II; Metastasis; Real-time quantitative PCR; Human gastric cancer; HGC-27 cells; Matrix metalloproteinases

Core tip: In this study, we retrospectively analyzed the expression of annexin II in 51 cases of gastric carcinoma and 24 cases of adjacent tissue to investigate the relationship between the positive expression of annexin II and the invasion and metastasis of gastric cancer.