Published online Jul 7, 2017. doi: 10.3748/wjg.v23.i25.4569
Peer-review started: February 9, 2017
First decision: March 16, 2017
Revised: March 27, 2017
Accepted: April 12, 2017
Article in press: April 12, 2017
Published online: July 7, 2017
Processing time: 149 Days and 18.6 Hours
To investigate the functional role and underlying molecular mechanism of miR-29a in hepatitis B virus (HBV) expression and replication.
The levels of miR-29a and SMARCE1 in HBV-infected HepG2.2.15 cells were measured by quantitative real-time PCR and western blot analysis. HBV DNA replication was measured by quantitative PCR and Southern blot analysis. The relative levels of hepatitis B surface antigen and hepatitis B e antigen were detected by enzyme-linked immunosorbent assay. The Cell Counting Kit-8 (CCK-8) was used to detect the viability of HepG2.2.15 cells. The relationship between miR-29a and SMARCE1 were identified by target prediction and luciferase reporter analysis.
miR-29a promoted HBV replication and expression, while SMARCE1 repressed HBV replication and expression. Cell viability detection indicated that miR-29a transfection had no adverse effect on the host cells. Moreover, SMARCE1 was identified and validated to be a functional target of miR-29a. Furthermore, restored expression of SMARCE1 could relieve the increased HBV replication and expression caused by miR-29a overexpression.
miR-29a promotes HBV replication and expression through regulating SMARCE1. As a potential regulator of HBV replication and expression, miR-29a could be a promising therapeutic target for patients with HBV infection.
Core tip: Although aberrant miR-29a expression has been found to be involved in the process of hepatitis B virus (HBV) infection, its underlying mechanism remains unclear. In this study, we analyzed the expression levels of miR-29a in HBV-infected HepG2.2.15 cells. Our results indicated that miR-29a expression was up-regulated in HBV-associated hepatocellular carcinoma cells. In addition, SMARCE1 was confirmed to be a functional target of miR-29a. miR-29a promoted HBV replication and expression through directly regulating SMARCE1. These findings provide potential therapeutic targets for patients with HBV infection.