Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 7, 2017; 23(1): 87-92
Published online Jan 7, 2017. doi: 10.3748/wjg.v23.i1.87
Hydrogen sulfide attenuates gastric mucosal injury induced by restraint water-immersion stress via activation of KATP channel and NF-κB dependent pathway
Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang
Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang, College of Life Science, Qi Lu Normal University, Zhangqiu 250200, Jinan, Shandong Province, China
Author contributions: Sun HZ and Zheng S contributed equally to this work; Sun HZ conceived and designed the experiments; Zheng S, Lu K, Hou FT, Bi JX, Liu XL and Wang SS performed the experiments; Sun HZ and Zheng S analyzed the data; Sun HZ contributed reagents/materials/analysis tools; Sun HZ wrote the paper.
Supported by Natural Science Foundation of Shandong Province, No. ZR2015CL016 and No. ZR2011CL012; and Colleges and Universities of Shandong Province Science and Technology Plan Projects, No. J11LC17.
Institutional review board statement: The study was reviewed and approved by the Qi Lu Normal University Institutional Review Board.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Qi Lu Normal University (IACUC protocol No. 14276).
Conflict-of-interest statement: To the best of our knowledge, no conflict of interest exists.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Hong-Zhao Sun, Associate Professor, College of Life Science, Qi Lu Normal University, No. 2, Wenbo Road, Zhangqiu 250200, Jinan, Shandong Province, China. sunhongzhao18@126.com
Telephone: +86-531-66778211 Fax: +86-531-66778037
Received: August 16, 2016
Peer-review started: August 16, 2016
First decision: October 11, 2016
Revised: October 19, 2016
Accepted: November 15, 2016
Article in press: November 16, 2016
Published online: January 7, 2017
Processing time: 142 Days and 7.2 Hours
Abstract
AIM

To explore the effect of hydrogen sulfide (H2S) on restraint water-immersion stress (RWIS)-induced gastric lesions in rats and the influence of adenosine triphosphate (ATP)-sensitive potassium (KATP) channels and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway on such an effect.

METHODS

Male Wistar rats were randomly divided into a control group, a physiological saline (PS) group, a sodium hydrosulfide (NaHS) group, a glibenclamide (Gl) group, Gl plus NaHS group, a pyrrolidine dithiocarbamate (PDTC) group, and a PDTC plus NaHS group. Gastric mucosal injury was induced by RWIS for 3 h in rats, and gastric mucosal damage was analyzed after that. The PS, NaHS (100 μmol/kg body weight), Gl (100 μmol/kg body weight), Gl (100 μmol/kg or 150 μmol/kg body weight) plus NaHS (100 μmol/kg body weight), PDTC (100 μmol/kg body weight), and PDTC (100 μmol/kg body weight) plus NaHS (100 μmol/kg body weight) were respectively injected intravenously before RWIS.

RESULTS

RWIS induced serious gastric lesions in the rats in the PS pretreatment group. The pretreatment of NaHS (a H2S donor) significantly reduced the damage induced by RWIS. The gastric protective effect of the NaHS during RWIS was attenuated by PDTC, an NF-κB inhibitor, and also by glibenclamide, an ATP-sensitive potassium channel blocker, in a dose-dependent manner.

CONCLUSION

These results suggest that exogenous H2S plays a protective role against RWIS injury in rats, possibly through modulation of KATP channel opening and the NF-κB dependent pathway.

Keywords: Hydrogen sulfide; Nuclear factor kappa B; Gastric mucosal injury; Restraint water-immersion stress; Adenosine triphosphate-sensitive potassium

Core tip: In this study, the authors demonstrate that exogenous hydrogen sulfide plays a protective role against restraint water-immersion stress injury in rats possibly through modulation of adenosine triphosphate-sensitive potassium channel opening and the nuclear factor kappa-light-chain-enhancer of activated B cells dependent pathway.