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©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
Antioxidant and anti-inflammatory action of melatonin in an experimental model of secondary biliary cirrhosis induced by bile duct ligation
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Francielli Licks, Mariana do Couto Soares, Adriane Dal Bosco, Norma Possa Marroni
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Adriane Dal Bosco, Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90040-060, Brazil
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Francielli Licks, Mariana do Couto Soares, Adriane Dal Bosco, Norma Possa Marroni, Laboratory of Experimental Hepatology and Gastroenterology, Hospital de Clínicas de Porto Alegre, Porto Alegre 90035-903, Brazil
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Francielli Licks, Mariana do Couto Soares, Adriane Dal Bosco, Norma Possa Marroni, Laboratory of Oxidative Stress and Antioxidants, Universidade Luterana do Brasil, Canoas 92425-900, Brazil
Francielli Licks, Graduate Program in Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre 90050-170, Brazil
Author contributions: Colares JR and Marroni NP participated in the study design and development, analysis and interpretation of data and writing of the article; Schemitt EG, Hartmann RM, Licks F, Soares MC and Bosco AD participated in the oxidative stress and immunohistochemistry analyses and the interpretation of results.
Supported by Research and Event Promotion (FIPE) end accomplished in Hospital de Clínicas de Porto Alegre, No. 14-0474.
Institutional review board statement: The study was realized and approved by the Ethics Committee of HCPA (No. 14-0474).
Institutional animal care and use committee statement: All collections of biological samples and analyses carried out were in accordance with ethical principles of the Committee for Ethics on Animal Use (CEUA-HCPA [No. 14-0474]).
Conflict-of-interest statement: To the best of our knowledge, no conflict of interest exists.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Josieli Raskopf Colares, MD, Cellular Biology and Molecular Applied to Health, Laboratory of Oxidative Stress and Antioxidants, Universidade Luterana do Brasil, Avenida Farroupilha 8001, Canoas 92425-900, Brazil. jozy.ma@hotmail.com
Telephone: +55-51-97516065 Fax: +55-51-33598760
Received: June 28, 2016
Peer-review started: June 29, 2016
First decision: August 8, 2016
Revised: August 24, 2016
Accepted: September 14, 2016
Article in press: September 14, 2016
Published online: October 28, 2016
AIM
To evaluate the effects of melatonin (Mel) on oxidative stress in an experimental model of bile duct ligation (BDL).
METHODS
Male Wistar rats (n = 32, weight ± 300 g) were allocated across four groups: CO (sham BDL), BDL (BDL surgery), CO + Mel (sham BDL and Mel administration) and BDL + Mel (BDL surgery and Mel administration). Mel was administered intraperitoneally for 2 wk, starting on postoperative day 15, at a dose of 20 mg/kg.
RESULTS
Mel was effective at the different standards, reestablishing normal liver enzyme levels, reducing the hepatosomatic and splenosomatic indices, restoring lipoperoxidation and antioxidant enzyme concentrations, reducing fibrosis and inflammation, and thereby reducing liver tissue injury in the treated animals.
CONCLUSION
The results of this study suggest a protective effect of Mel when administered to rats with secondary biliary cirrhosis induced by BDL.
Core tip: Secondary biliary cirrhosis is a late complication of prolonged extrahepatic bile duct obstruction that leads to structural and functional changes in the liver. Melatonin, the main product of the pineal gland, provides hepatic protection in the experimental model of bile duct ligation.
