Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 14, 2016; 22(14): 3746-3757
Published online Apr 14, 2016. doi: 10.3748/wjg.v22.i14.3746
S-adenosyl-L-methionine modifies antioxidant-enzymes, glutathione-biosynthesis and methionine adenosyltransferases-1/2 in hepatitis C virus-expressing cells
Sonia Amelia Lozano-Sepulveda, Eduardo Bautista-Osorio, Jose Angel Merino-Mascorro, Marta Varela-Rey, Linda Elsa Muñoz-Espinosa, Paula Cordero-Perez, María Luz Martinez-Chantar, Ana Maria Rivas-Estilla
Sonia Amelia Lozano-Sepulveda, Eduardo Bautista-Osorio, Jose Angel Merino-Mascorro, Ana Maria Rivas-Estilla, Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Nuevo Leon 64460, Mexico
Marta Varela-Rey, María Luz Martinez-Chantar, Metabolomic Unit, CIC bioGUNE, CIBERehd, Parque Tecnológico de Bizkaia, 48160 Derio, Spain
Linda Elsa Muñoz-Espinosa, Paula Cordero-Perez, Liver Unit, School of Medicine and Hospital Universitario, Autonomous University of Nuevo Leon, Monterrey, Nuevo Leon 64460, Mexico
Author contributions: Lozano-Sepulveda SA performed the majority of experiments, analyzed the data and partially wrote the paper; Bautista-Osorio E, Varela-Rey M, Martinez-Chantar ML and Merino-Mascorro JA performed additional molecular investigations and analyzed data; Muñoz-Espinosa L and Cordero-Perez P partially designed and coordinated the research; Rivas-Estilla AM designed, coordinated the research and wrote the paper.
Supported by CONACYT-Mexico, grant register CB2010-01-155082 to Rivas-Estilla AM.
Institutional review board statement: In this study, we performed all experiments in vitro using cell lines and did not include humans or animals; therefore this requirement does not apply.
Conflict-of-interest statement: All listed authors in this manuscript do not have financial relationships to disclose.
Data sharing statement: We did not include data sharing from other researchers.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Ana Maria Rivas-Estilla, Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Francisco I. Madero y Ave. Gonzalitos s/n Col. Mitras Centro, Monterrey, Nuevo Leon 64460, Mexico. amrivas1@yahoo.ca
Telephone: +52-81-83337747
Received: December 26, 2015
Peer-review started: December 28, 2015
First decision: January 28, 2016
Revised: February 14, 2016
Accepted: March 1, 2016
Article in press: March 2, 2016
Published online: April 14, 2016
Processing time: 94 Days and 9.1 Hours
Abstract

AIM: To elucidate the mechanism(s) by which S-adenosyl-L-methionine (SAM) decreases hepatitis C virus (HCV) expression.

METHODS: We examined the effects of SAM on viral expression using an HCV subgenomic replicon cell culture system. Huh7 HCV-replicon cells were treated with 1 mmol/L SAM for different times (24-72 h), then total RNA and proteins were isolated. cDNA was synthesized and real time-PCR was achieved to quantify HCV-RNA, superoxide dismutase 1 and 2 (SOD-1, SOD-2) catalase, thioredoxin 1, methionine adenosyltransferase 1A and 2A (MAT1A, MAT2A) expression, and GAPDH and RPS18 as endogenous genes. Expression of cellular and viral protein was evaluated by western-blot analysis using antibodies vs HCV-NS5A, SOD-1, SOD-2, catalase, thioredoxin-1, MAT1A, MAT2A, GAPDH and actin. Total glutathione levels were measured at different times by Ellman’s recycling method (0-24 h). Reactive oxidative species (ROS) levels were quantified by the dichlorofluorescein assay (0-48 h); Pyrrolidin dithiocarbamate (PDTC) was tested as an antioxidant control and H2O2 as a positive oxidant agent.

RESULTS: SAM exposition decreased HCV-RNA levels 50%-70% compared to non-treated controls (24-72 h). SAM induced a synergic antiviral effect with standard IFN treatment but it was independent of IFN signaling. In addition, 1 mmol/L SAM exposition did not modify viral RNA stability, but it needs cellular translation machinery in order to decrease HCV expression. Total glutathione levels increased upon SAM treatment in HCV-replicon cells. Transcriptional antioxidant enzyme expression (SOD-1, SOD-2 and thioredoxin-1) was increased at different times but interestingly, there was no significant change in ROS levels upon SAM treatment, contrary to what was detected with PDTC treatment, where an average 40% reduction was observed in exposed cells. There was a turnover from MAT1A/MAT2A, since MAT1A expression was increased (2.5 fold-times at 48 h) and MAT2A was diminished (from 24 h) upon SAM treatment at both the transcriptional and translational level.

CONCLUSION: A likely mechanism(s) by which SAM diminish HCV expression could involve modulating antioxidant enzymes, restoring biosynthesis of glutathione and switching MAT1/MAT2 turnover in HCV expressing cells.

Keywords: Hepatitis C virus; S-adenosyl-L-methionine; Superoxide dismutase 1; Superoxide dismutase 2; Replication; Hepatitis C virus-RNA; NS5A; Oxidative stress; Antioxidants; Viral proteins; Reactive oxygen species; Pyrrolidine dithiocarbamate

Core tip: S-adenosyl-L-methionine (SAM) downregulates hepatitis C virus (HCV) expression by unknown mechanisms. We evaluated the effects of SAM on viral expression using an HCV subgenomic replicon cell culture system. We observed that SAM induces a synergic antiviral effect with standard interferon (IFN) treatment independently of IFN signaling pathways; it does not modify viral RNA stability, but it needs cellular translation machinery in order to decrease HCV expression. In addition, results demonstrated that a likely mechanism(s) by which SAM decreases HCV expression could involve modulating antioxidant enzyme systems, restoring biosynthesis of glutathione and switching methionine adenosyltransferase 1 (MAT1)/MAT2 turnover in HCV expressing cells.