Case Control Study
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World J Gastroenterol. Jun 21, 2014; 20(23): 7466-7472
Published online Jun 21, 2014. doi: 10.3748/wjg.v20.i23.7466
Association of MYO9B gene polymorphisms with inflammatory bowel disease in Chinese Han population
Jing Hu, Qiao Mei, Jian Huang, Nai-Zhong Hu, Xiao-Chang Liu, Jian-Ming Xu
Jing Hu, Qiao Mei, Jian Huang, Nai-Zhong Hu, Xiao-Chang Liu, Jian-Ming Xu, Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Key Laboratory of Gastroenterology of Anhui Province, Hefei 230022, Anhui Province, China
Author contributions: Hu J, Mei Q, Hu NZ and Xu JM designed the research; Hu J and Huang J performed the research; Huang J and Liu XC analyzed the data; Hu J wrote the paper; Xu JM revised the manuscript.
Correspondence to: Jian-Ming Xu, PhD, Professor, Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Key Laboratory of Gastroenterology of Anhui Province, Jixi Rd 218, Hefei 230022, Anhui Province, China. xujm1017@163.com
Telephone: +86-551-62922039 Fax: +86-551-63633742
Received: January 16, 2014
Revised: March 15, 2014
Accepted: April 21, 2014
Published online: June 21, 2014
Processing time: 160 Days and 1.4 Hours
Abstract

AIM: To explore the association of MYO9B gene polymorphisms with clinical phenotypes and intestinal permeability of individuals with inflammatory bowel disease (IBD) in China.

METHODS: A total of 442 IBD patients and 402 healthy volunteers were genotyped for two single nucleotides (rs962917 and rs1545620) using the ligase detection reaction and polymerase chain reaction. Allelic and genotype frequency analyses were performed for the two groups. Intestinal permeability was evaluated using lactulose (L) and mannitol (M) excretion. The association of MYO9B gene polymorphisms with intestinal permeability between the normal and high intestinal permeability groups was analyzed.

RESULTS: Overall, there was no significant difference in the genotypic and allelic frequencies of MYO9B between IBD patients and controls. Although no association was found with ulcerative colitis in the comparison between the subgroups, the frequencies of rs962917 and rs1545620 were different in the Crohn’s disease (CD) subgroup with ileocolitis (CC vs CT and TT, P = 0.014; and AA vs AC and CC, P = 0.022, respectively). rs1545620 variants appear to be the genetic susceptibility factor for perianal disease in CD patients (AA vs AC CC, P = 0.029). In addition, the L/M ratio was significantly higher in IBD patients than in controls (0.065 ± 0.013 vs 0.020 ± 0.002, P = 0.02), but no association was found between the MYO9B gene and the L/M ratio in IBD patients.

CONCLUSION: MYO9B gene polymorphisms may influence the sub-phenotypic expression of CD in China. No association between these MYO9B polymorphisms and intestinal permeability in IBD patients was found.

Keywords: Inflammatory bowel disease; Crohn’s disease; Ulcerative colitis; MYO9B; Genetic susceptibility; Intestinal permeability

Core tip: An association between MYO9B gene polymorphisms and inflammatory bowel disease (IBD) in the Chinese Han population has not yet been confirmed. The authors aimed to explore the association of MYO9B gene polymorphisms with the clinical phenotypes and intestinal permeability of IBD in China. The results suggested that MYO9B gene polymorphisms may influence the sub-phenotypic expression of Crohn’s disease but failed to confirm an association between the MYO9B polymorphisms and intestinal permeability in Chinese Han IBD. These findings indicate that the MYO9B gene may differ among IBD patients of various races from various regions.