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World J Gastroenterol. May 28, 2014; 20(20): 5962-5972
Published online May 28, 2014. doi: 10.3748/wjg.v20.i20.5962
miR-106b-25/miR-17-92 clusters: Polycistrons with oncogenic roles in hepatocellular carcinoma
Weiqi Tan, Yang Li, Seng-Gee Lim, Theresa MC Tan
Weiqi Tan, Theresa MC Tan, Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore S117597, Singapore
Yang Li, Seng-Gee Lim, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore S117597, Singapore
Author contributions: All authors contributed equally to the paper.
Correspondence to: Dr. Theresa MC Tan, Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, MD7, 8 Medical Drive, Singapore S117597, Singapore. bchtant@nus.edu.sg
Telephone: +65-1-65163685 Fax: +65-1-67791453
Received: October 27, 2013
Revised: January 11, 2014
Accepted: April 1, 2014
Published online: May 28, 2014
Processing time: 212 Days and 18.4 Hours
Abstract

MicroRNAs are small endogenously expressed RNA molecules which are involved in the process of silencing gene expression through translational regulation. The polycistronic miR-17-92 cluster is the first microRNA cluster shown to play a role in tumorigenesis. It has two other paralogs in the human genome, the miR-106b-25 cluster and the miR-106a-363 cluster. Collectively, the microRNAs encoded by these clusters can be further grouped based on the seed sequences into four families, namely the miR-17, the miR-92, the miR-18 and the miR-19 families. Over-expression of the miR-106b-25 and miR-17-92 clusters has been reported not only during the development of cirrhosis but also subsequently during the development of hepatocellular carcinoma. Members of these clusters have also been shown to affect the replication of hepatitis B and hepatitis C viruses. Various targets of these microRNAs have been identified, and these targets are involved in tumor growth, cell survival and metastasis. In this review, we first describe the regulation of these clusters by c-Myc and E2F1, and how the members of these clusters in turn regulate E2F1 expression forming an auto-regulatory loop. In addition, the roles of the various members of the clusters in affecting relevant target gene expression in the pathogenesis of hepatocellular carcinoma will also be discussed.

Keywords: miR-106b-25 cluster; miR-17-92 cluster; Hepatocellular carcinoma; Liver cirrhosis; MicroRNAs

Core tip: The polycistronic miR-17-92 cluster has been characterized to play a role in tumorigenesis. Over-expression of the miR-17-92 cluster and its paralog, the miR-106b-25 cluster, has been reported in the development of cirrhosis and hepatocellular carcinoma. Various targets of these microRNAs have been identified, and these targets are involved in tumor growth, cell survival and metastasis. We describe the regulation of these clusters by c-Myc and E2F1, and discuss the roles of the various members of the clusters in affecting relevant target gene expression in the pathogenesis of hepatocellular carcinoma.