Brief Article
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World J Gastroenterol. Jan 28, 2013; 19(4): 575-580
Published online Jan 28, 2013. doi: 10.3748/wjg.v19.i4.575
Increased expression of tyrosine phosphatase SHP-2 in Helicobacter pylori-infected gastric cancer
Jing Jiang, Mei-Shan Jin, Fei Kong, Yin-Ping Wang, Zhi-Fang Jia, Dong-Hui Cao, Hong-Xi Ma, Jian Suo, Xue-Yuan Cao
Jing Jiang, Fei Kong, Zhi-Fang Jia, Dong-Hui Cao, Division of Clinical Epidemiology, Jilin University First Hospital, Changchun 130021, Jilin Province, China
Mei-Shan Jin, Yin-Ping Wang, Hong-Xi Ma, Division of Pathology, Jilin University First Hospital, Changchun 130021, Jilin Province, China
Jian Suo, Xue-Yuan Cao, Department of Gastric and Colorectal Surgery, Jilin University First Hospital, Changchun 130021, Jilin Province, China
Author contributions: Cao XY and Jiang J designed the study and carried out most of the study; Jiang J, Cao XY and Jin MS wrote the first draft of manuscript; Jin MS, Wang YP and Kong F analyzed pathological data; Kong F, Jia ZF, Cao DH collected patients’ clinical information; Jiang J performed statistical analysis; Ma HX and Suo J provided valuable suggestions in the preparation of the manuscript.
Supported by National Natural Science Foundation of China, No. 81072369; and Natural Science Foundation of Jilin Province, China, No. 200905131
Correspondence to: Xue-Yuan Cao, MD, PhD, Department of Gastric and Colorectal Surgery, Jilin University First Hospital, 71th Xin Min Street, Changchun 130021, Jilin Province, China. ccmzc32jdyycao@yahoo.com.cn
Telephone: +86-431-88783953 Fax: +86-431-88782889
Received: October 16, 2012
Revised: November 17, 2012
Accepted: December 15, 2012
Published online: January 28, 2013
Processing time: 105 Days and 5.9 Hours
Abstract

AIM: To explore the alteration of tyrosine phosphatase SHP-2 protein expression in gastric cancer and to assess its prognostic values.

METHODS: Three hundred and five consecutive cases of gastric cancer were enrolled into this study. SHP-2 expression was carried out in 305 gastric cancer specimens, of which 83 were paired adjacent normal gastric mucus samples, using a tissue microarray immunohistochemical method. Correlations were analyzed between expression levels of SHP-2 protein and tumor parameters or clinical outcomes. Serum anti-Helicobacter pylori (H. pylori) immunoglobulin G was detected with enzyme-linked immunosorbent assay. Cox proportional hazards model was used to evaluate prognostic values by compassion of the expression levels of SHP-2 and disease-specific survivals in patients.

RESULTS: SHP-2 staining was found diffuse mainly in the cytoplasm and the weak staining was also observed in the nucleus in gastric mucosa cells. Thirty-two point five percent of normal epithelial specimen and 62.6% of gastric cancer specimen were identified to stain with SHP-2 antibody positively (P < 0.001). Though SHP-2 staining intensities were stronger in the H. pylori (+) group than in the H. pylori (-) group, no statistically significant difference was found in the expression levels of SHP-2 between H. pylori (+) and H. pylori (-) gastric cancer (P = 0.40). The SHP-2 expression in gastric cancer was not significantly associated with cancer stages, lymph node metastases, and distant metastasis of the tumors (P = 0.34, P = 0.17, P = 0.52). Multivariate analysis demonstrated no correlation between SHP-2 expression and disease-free survival (P = 0.86).

CONCLUSION: Increased expression of SHP-2 protein in gastric cancer specimen suggesting the aberrant up-regulation of SHP-2 protein might play an important role in the gastric carcinogenesis.

Keywords: Gastric cancer; SH2-containing protein tyrosine phosphatase 2; Expression; Helicobacter pylori