Original Article
Copyright ©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Mar 28, 2013; 19(12): 1912-1918
Published online Mar 28, 2013. doi: 10.3748/wjg.v19.i12.1912
Expression and clinical significance of CD73 and hypoxia-inducible factor-1α in gastric carcinoma
Xiao-Xia Lu, Yi-Tian Chen, Bing Feng, Xiao-Bei Mao, Bo Yu, Xiao-Yuan Chu
Xiao-Xia Lu, Yi-Tian Chen, Bing Feng, Xiao-Bei Mao, Xiao-Yuan Chu, Department of Medical Oncology, Nanjing General Hospital of Nanjing Military Command, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China
Bo Yu, Department of Pathology, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, Jiangsu Province, China
Author contributions: Lu XX and Chen YT contributed equally to this work; Lu XX and Chu XY designed the research; Chen YT, Mao XB and Yu B performed the research; Lu XX and Chen YT evaluated data and prepared the manuscript; Lu XX, Feng B and Chu XY were involved in the manuscript writing.
Supported by National Natural Science Foundation of China, No. 81071806
Correspondence to: Xiao-Yuan Chu, Associate Professor, Department of Medical Oncology, Nanjing General Hospital of Nanjing Military Command, Medical School of Nanjing University, No. 305 Zhongshan Road, Nanjing 210002, Jiangsu Province, China. chuxiaoyuan6906@gmail.com
Telephone: +86-25-80860072 Fax: +86-25-80860072
Received: July 15, 2012
Revised: August 15, 2012
Accepted: September 28, 2012
Published online: March 28, 2013
Abstract

AIM: To investigate the expression of CD73 and hypoxia-inducible factor-1α (HIF-1α) in human gastric carcinoma, and explore their clinical significance and prognostic value.

METHODS: CD73 and HIF-1α expressions were detected by immunohistochemistry in consecutive sections of tissue samples from 68 gastric carcinoma patients. The peritumor tissues 2 cm away from the tumor were obtained and served as controls. The presence of CD73 and HIF-1α was analyzed by immunohistochemistry using the Envision technique.

RESULTS: CD73 and HIF-1α expressions in gastric carcinoma were significantly higher than those in gastric mucosal tissues as control (P < 0.001) and showed a close correlation (Spearman r = 0.390, P = 0.001). Overexpression of CD73 was positively correlated with differentiation of tumor (P = 0.000), histopathology (P = 0.041), depth of invasion (P < 0.001), nodal status (P = 0.003), metastasis (P = 0.013), and the American Joint Committee on Cancer (AJCC) stage (P < 0.001). High expression of HIF-1α was positively correlated with tumor diameter (P = 0.031), depth of invasion (P = 0.022), and AJCC stage (P = 0.035). The overall survival rate was low in the patients with high expression of CD73 (P < 0.001). Moreover, CD73+/HIF-1α+ patients had the worst prognosis (P < 0.001). CD73 expression was proven to be an independent predictor for patients with gastric carcinoma by both multivariate Cox regression analysis (P = 0.021) and receiver operating characteristic curves (P = 0.001).

CONCLUSION: CD73 expression correlates closely with HIF-1α expression in gastric carcinoma. CD73 could be an independent prognostic indicator for gastric carcinoma.

Keywords: CD73, Hypoxia-inducible factor-1α, Gastric carcinoma, Immunohistochemistry, Prognosis