Kuai WX, Wang Q, Yang XZ, Zhao Y, Yu R, Tang XJ. Interleukin-8 associates with adhesion, migration, invasion and chemosensitivity of human gastric cancer cells. World J Gastroenterol 2012; 18(9): 979-985 [PMID: 22408359 DOI: 10.3748/wjg.v18.i9.979]
Corresponding Author of This Article
Xiao-Jun Tang, Associate professor, Department of Gastrointestinal Surgery, Huaian First Hospital affiliated to Nanjing Medical University, Huaian 223300, Jiangsu Province, China. txj9743@163.com
Article-Type of This Article
Brief Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Wen-Xia Kuai, Department of Pediatrics, Huaian First Hospital Affiliated to Nanjing Medical University, Huaian 223300, Jiangsu Province, China
Qiong Wang, Xiao-Zhong Yang, Department of Digestion, Huaian First Hospital Affiliated to Nanjing Medical University, Huaian 223300, Jiangsu Province, China
Yao Zhao, Ren Yu, Xiao-Jun Tang, Department of Gastrointestinal Surgery, Huaian First Hospital Affiliated to Nanjing Medical University, Huaian 223300, Jiangsu Province, China
Author contributions: Kuai WX, Wang Q contributed equally to this work; Kuai WX, Wang Q, Yang XZ, Zhao Y, Yu R, Tang XJ designed research; Kuai WX, Wang Q performed research; Yang XZ, Zhao Y provided new reagents/analytic tools; Yu R analyzed data; and Kuai WX, Wan Q and Tang XJ wrote the paper.
Supported by The Fund of Nanjing Medical University Science and Technology Development, No. 09NJMUZ30
Correspondence to: Xiao-Jun Tang, Associate professor, Department of Gastrointestinal Surgery, Huaian First Hospital affiliated to Nanjing Medical University, Huaian 223300, Jiangsu Province, China. txj9743@163.com
Telephone: +86-517-80272314 Fax: +86-517-84922412
Received: June 23, 2011 Revised: September 30, 2011 Accepted: December 31, 2011 Published online: March 7, 2012
Abstract
AIM: To investigate the relationship between Interleukin-8 (IL-8) and proliferation, adhesion, migration, invasion and chemosensitivity of gastric cancer (GC) cells.
METHODS: The IL-8 cDNA was stably transfected into human GC cell line MKN-45 and selected IL-8-secreting transfectants. The expression of IL-8 in human GC cell line KATO-III was inhibited by RNA interference. The expressions of mRNA and protein of IL-8 in GC cells were detected by real-time reverse transcription-polymerase chain reaction or enzyme-linked immunosorbent assay (ELISA).
RESULTS: The overexpression of IL-8 resulted in an increased cell adhesion, migration and invasion, and a significant resistance to oxaliplatin in MKN-45 cells. Inhibition of IL-8 expression with small interfering RNA decreased the adhesion, migration and invasion functions and oxaliplatin resistance in KATO-III cells. IL-8 increased NF-κB and Akt activities and adhesion molecules ICAM-1, VCAM-1, and CD44 expression in GC cells.
CONCLUSION: Overexpression of IL-8 promotes the adhesion, migration, invasion, and chemoresistance of GC cells, indicating that IL-8 is an important therapeutic target in GC.
