Weigand K, Voigt F, Encke J, Hoyler B, Stremmel W, Eisenbach C. Vaccination with dendritic cells pulsed with hepatitis C pseudo particles induces specific immune responses in mice. World J Gastroenterol 2012; 18(8): 785-793 [PMID: 22371638 DOI: 10.3748/wjg.v18.i8.785]
Corresponding Author of This Article
Dr. Kilian Weigand, Department of Gastroenterology and Hepatology, Medizinische Klinik IV, University Hospital Heidelberg, Im Neuenheimer Feld 410, D-69120 Heidelberg, Germany. kilian_weigand@med.uni-heidelberg.de
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World J Gastroenterol. Feb 28, 2012; 18(8): 785-793 Published online Feb 28, 2012. doi: 10.3748/wjg.v18.i8.785
Vaccination with dendritic cells pulsed with hepatitis C pseudo particles induces specific immune responses in mice
Kilian Weigand, Franziska Voigt, Jens Encke, Birgit Hoyler, Wolfgang Stremmel, Christoph Eisenbach
Kilian Weigand, Franziska Voigt, Birgit Hoyler, Wolfgang Stremmel, Christoph Eisenbach, Department of Gastroenterology and Hepatology, University Hospital Heidelberg, D-69120 Heidelberg, Germany
Jens Encke, Department of Medicine, Johanna-Etienne-Krankenhaus Neuss, 41462 Neuss, Germany
Author contributions: Weigand K, Voigt F and Eisenbach C performed the majority of experiments; Weigand K and Encke J designed the study; Hoyler B and Eisenbach C provided vital reagents and gave important technical support; Stremmel W co-ordinated the research group in addition to providing financial support; Encke J and Eisenbach C were involved in editing the manuscript; and Weigand K wrote the manuscript.
Correspondence to: Dr. Kilian Weigand, Department of Gastroenterology and Hepatology, Medizinische Klinik IV, University Hospital Heidelberg, Im Neuenheimer Feld 410, D-69120 Heidelberg, Germany. kilian_weigand@med.uni-heidelberg.de
Telephone: +49-6221-5638747 Fax: +49-6221-565255
Received: March 3, 2011 Revised: March 26, 2011 Accepted: June 13, 2011 Published online: February 28, 2012
Abstract
AIM: To explore dendritic cells (DCs) multiple functions in immune modulation.
METHODS: We used bone-marrow derived dendritic cells from BALB/c mice pulsed with pseudo particles from the hepatitis C virus to vaccinate naive BALB/c mice. Hepatitis C virus (HCV) pseudo particles consist of the genotype 1b derived envelope proteins E1 and E2, covering a non-HCV core structure. Thus, not a single epitope, but the whole “viral surface” induces immunogenicity. For vaccination, mature and activated DC were injected subcutaneously twice.
RESULTS: Humoral and cellular immune responses measured by enzyme-linked immunosorbent assay and interferon-gamma enzyme-linked immunosorbent spot test showed antibody production as well as T-cells directed against HCV. Furthermore, T-cell responses confirmed two highly immunogenic regions in E1 and E2 outside the hypervariable region 1.
CONCLUSION: Our results indicate dendritic cells as a promising vaccination model for HCV infection that should be evaluated further.
