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World J Gastroenterol. Nov 28, 2012; 18(44): 6379-6386
Published online Nov 28, 2012. doi: 10.3748/wjg.v18.i44.6379
Dietary supplementation of some antioxidants against hypoxia
Sanaa Ahmed Ali, Hanan Farouk Aly, Lilla Mohammed Faddah, Zeenat F Zaidi
Sanaa Ahmed Ali, Hanan Farouk Aly, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries, National Research Center, Cairo 12622, Egypt
Lilla Mohammed Faddah, Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia
Zeenat F Zaidi, Department of Anatomy, Faculty Of Medicine, King Saud University, Riyadh 11495, Saudi Arabia
Author contributions: Ali SA and Aly HF did the biochemical and histological analysis, performed the statistical analysis and wrote the paper; Faddah LM and Zaidi ZF designed the experimental model and participated in the histological analysis.
Correspondence to: Sanaa Ahmed Ali, Professor, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries, National Research Center, El-Behooth St. 31, Dokki, Cairo 12622, Egypt. sanaa_ahmedibrahim@yahoo.com
Telephone: +20-201-3394667 Fax: +20-233-370931
Received: April 19, 2012
Revised: July 19, 2012
Accepted: July 28, 2012
Published online: November 28, 2012
Abstract

The present study aims to clarify the protective effect of supplementation with some antioxidants, such as idebenone (200 mg/kg, ip), melatonin (10 mg/kg, ip) and arginine (200 mg/kg, ip) and their combination, on liver function (T. protein, albumin, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase), energetic parameters (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, inorganic phosphate, total adenylate, adenylate energy charge and potential phosphate). The effect on glycolytic and glycogenolytic enzymes (glucose, glycogen, glycogen phosphorylase, pyruvate kinase and phosphofructokinase against hypoxia) was also studied. The drugs were administered 24 and 1 h prior sodium nitrite intoxication. All biochemical parameters were estimated 1 h after sodium nitrite injection. Injection of sodium nitrite (75 mg/kg, sc) produced a significant disturbance in all biochemical parameters of liver function, energetic parameters and glycolytic and glycogenolytic enzymes. Hepatic damage was confirmed by histopathological examination of the liver as compared to controls. The marked changes in hepatic cells induced by sodium nitrite were completely abolished by pretreatment with the drug combination, suggesting potential protection against sodium nitrite-induced hypoxia. It could be concluded that a combination of both idebenone and melatonin or idebenone and arginine provides potential protection against sodium nitrite-induced hypoxia by improving biochemical parameters and preserving liver histology.

Keywords: Hypoxia; Idebenone; Melatonin; Nitrate/nitrite; Adenosine triphosphate