Salamone F, Li Volti G, Titta L, Puzzo L, Barbagallo I, La Delia F, Zelber-Sagi S, Malaguarnera M, Pelicci PG, Giorgio M, Galvano F. Moro orange juice prevents fatty liver in mice. World J Gastroenterol 2012; 18(29): 3862-3868 [PMID: 22876038 DOI: 10.3748/wjg.v18.i29.3862]
Corresponding Author of This Article
Federico Salamone, MD, Department of Internal Medicine, University of Catania, Via Santa Sofia, 78, 95123 Catania, Italy. federicosalamone@yahoo.it
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Brief Article
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World J Gastroenterol. Aug 7, 2012; 18(29): 3862-3868 Published online Aug 7, 2012. doi: 10.3748/wjg.v18.i29.3862
Moro orange juice prevents fatty liver in mice
Federico Salamone, Giovanni Li Volti, Lucilla Titta, Lidia Puzzo, Ignazio Barbagallo, Francesco La Delia, Shira Zelber-Sagi, Michele Malaguarnera, Pier Giuseppe Pelicci, Marco Giorgio, Fabio Galvano
Federico Salamone, Department of Internal Medicine, University of Catania, 95123 Catania, Italy
Giovanni Li Volti, Ignazio Barbagallo, Francesco La Delia, Michele Malaguarnera, Fabio Galvano, Department of Drug Sciences, University of Catania, 95125 Catania, Italy
Lucilla Titta, Pier Giuseppe Pelicci, Marco Giorgio, Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
Lidia Puzzo, Department of Pathology, University of Catania, 95123 Catania, Italy
Shira Zelber-Sagi, Department of Gastroenterology, Tel Aviv Sourasky Medical Center, Tel-Aviv 64239, Israel
Author contributions: Salamone F designed the study, performed the experiments, analyzed the data and wrote the manuscript; Li Volti G performed the experiments, analyzed the data and critically reviewed the manuscript; Titta L designed the study, performed the experiments, analyzed the data and critically reviewed the manuscript; Puzzo L, Barbagallo I and La Delia F performed the experiments and analyzed the data; Zelber-Sagi S and Pelicci PG critically reviewed the manuscript; Malaguarnera M performed the experiments, analyzed the data and critically reviewed the manuscript; Giorgio M designed the study, performed the experiments, analyzed the data and critically reviewed the manuscript; Galvano F designed the study, analyzed the data and contributed to writing of the manuscript.
Correspondence to: Federico Salamone, MD, Department of Internal Medicine, University of Catania, Via Santa Sofia, 78, 95123 Catania, Italy. federicosalamone@yahoo.it
Telephone: +39-320-6990366 Fax: +39-095-7384220
Received: October 29, 2011 Revised: February 16, 2012 Accepted: April 10, 2012 Published online: August 7, 2012
Abstract
AIM: To establish if the juice of Moro, an anthocyanin-rich orange, may improve liver damage in mice with diet-induced obesity.
METHODS: Eight-week-old mice were fed a high-fat diet (HFD) and were administrated water or Moro juice for 12 wk. Liver morphology, gene expression of lipid transcription factors, and metabolic enzymes were assessed.
RESULTS: Mice fed HFD displayed increased body weight, insulin resistance and dyslipidemia. Moro juice administration limited body weight gain, enhanced insulin sensitivity, and decreased serum triglycerides and total cholesterol. Mice fed HFD showed liver steatosis associated with ballooning. Dietary Moro juice markedly improved liver steatosis by inducing the expression of peroxisome proliferator-activated receptor-α and its target gene acylCoA-oxidase, a key enzyme of lipid oxidation. Consistently, Moro juice consumption suppressed the expression of liver X receptor-α and its target gene fatty acid synthase, and restored liver glycerol-3-phosphate acyltransferase 1 activity.
CONCLUSION: Moro juice counteracts liver steatogenesis in mice with diet-induced obesity and thus may represent a promising dietary option for the prevention of fatty liver.
