Leal MF, Calcagno DQ, Demachki S, Assumpção PP, Chammas R, Burbano RR, Smith MAC. Clinical implication of 14-3-3 epsilon expression in gastric cancer. World J Gastroenterol 2012; 18(13): 1531-1537 [PMID: 22509086 DOI: 10.3748/wjg.v18.i13.1531]
Corresponding Author of This Article
Mariana Ferreira Leal, PhD, Genetics Division, Department of Morphology and Genetic, Federal University of São Paulo, R. Botucatu, 740, São Paulo 04023-900, Brazil. mariana.morf@epm.br
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World J Gastroenterol. Apr 7, 2012; 18(13): 1531-1537 Published online Apr 7, 2012. doi: 10.3748/wjg.v18.i13.1531
Clinical implication of 14-3-3 epsilon expression in gastric cancer
Mariana Ferreira Leal, Danielle Queiroz Calcagno, Sâmia Demachki, Paulo Pimentel Assumpção, Roger Chammas, Rommel Rodríguez Burbano, Marília de Arruda Cardoso Smith
Mariana Ferreira Leal, Danielle Queiroz Calcagno, Marília de Arruda Cardoso Smith, Genetics Division, Department of Morphology and Genetic, Federal University of São Paulo, São Paulo 04023-900, Brazil
Sâmia Demachki, Pathology Service, João de Barros Barreto University Hospital, Federal University of Pará, Belém 60673-000, Brazil
Paulo Pimentel Assumpção, Surgery Service, João de Barros Barreto University Hospital, Federal University of Pará, Belém 60673-000, Brazil
Roger Chammas, Laboratory of Experimental Oncology, School of Medicine, University of São Paulo, São Paulo 01246-903, Brazil
Rommel Rodríguez Burbano, Human Cytogenetics Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém 66073-000, Brazil
Author contributions: Chammas R, Burbano RR and Smith MAC designed the study; Leal MF was involved in literature searches, and genetic and statistical analysis; Leal MF and Calcagno DQ were involved in data collection; Demachki S was responsible for pathological analysis; Assumpção PP was responsible for sample collection; Leal MF wrote the first draft of the manuscript; all authors listed have contributed to all subsequent drafts, and have approved the final manuscript.
Supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Chammas R, Smith MAC and Burbano RR) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; Leal MF and Calcagno DQ)
Correspondence to: Mariana Ferreira Leal, PhD, Genetics Division, Department of Morphology and Genetic, Federal University of São Paulo, R. Botucatu, 740, São Paulo 04023-900, Brazil. mariana.morf@epm.br
Telephone: +55-11-55764260 Fax: +55-11-55764260
Received: May 24, 2011 Revised: December 23, 2011 Accepted: December 31, 2011 Published online: April 7, 2012
Abstract
AIM: To evaluate for the first time the protein and mRNA expression of 14-3-3ε in gastric carcinogenesis.
METHODS: 14-3-3ε protein expression was determined by western blotting, and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue samples.
RESULTS: Authors observed a significant reduction of 14-3-3ε protein expression in gastric cancer (GC) samples compared to their matched non-neoplastic tissue. Reduced levels of 14-3-3ε were also associated with diffuse-type GC and early-onset of this pathology. Our data suggest that reduced 14-3-3ε may have a role in gastric carcinogenesis process.
CONCLUSION: Our results reveal that the reduced 14-3-3ε expression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcinogenesis process.
