Oksaharju A, Kankainen M, Kekkonen RA, Lindstedt KA, Kovanen PT, Korpela R, Miettinen M. Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells. World J Gastroenterol 2011; 17(6): 750-759 [PMID: 21390145 DOI: 10.3748/wjg.v17.i6.750]
Corresponding Author of This Article
Anna Oksaharju, MSc, Wihuri Research Institute, Kalliolinnantie 4, Helsinki 00140, Finland. anna.oksaharju@helsinki.fi
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Brief Article
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World J Gastroenterol. Feb 14, 2011; 17(6): 750-759 Published online Feb 14, 2011. doi: 10.3748/wjg.v17.i6.750
Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells
Anna Oksaharju, Matti Kankainen, Riina A Kekkonen, Ken A Lindstedt, Petri T Kovanen, Riitta Korpela, Minja Miettinen
Anna Oksaharju, Petri T Kovanen, Wihuri Research Institute, Helsinki 00140, Finland
Matti Kankainen, VTT Technical Research Centre of Finland, Biosystems Modelling, Espoo 02044, Finland
Riina A Kekkonen, Riitta Korpela, Valio Ltd, Research Centre, Helsinki 00370, Finland
Riina A Kekkonen, Riitta Korpela, University of Helsinki, Institute of Biomedicine, Medical Nutrition Physiology, Helsinki 00290, Finland
Ken A Lindstedt, Wihuri Research Institute, Helsinki 00140, Finland and Orion Corporation, Orion Pharma, Nonclinical R&D, Espoo 02601, Finland
Minja Miettinen, National Institute for Health and Welfare, Department of Vaccines and Immune Protection, Helsinki 00580, Finland
Author contributions: Kekkonen RA, Korpela R, Lindstedt KA and Oksaharju A designed the research; Oksaharju A performed the experiments; Oksaharju A, Kankainen M and Miettinen M analyzed the data; Oksaharju A wrote the manuscript; Kankainen M, Kekkonen RA, Lindstedt KA, Kovanen PT, Korpela R and Miettinen M edited the manuscript.
Supported by Valio Research Centre, the Foundation for Nutrition Research, Academy of Finland Research Council for Biosciences and Environment, Grant No. 129954; and Finnish Funding Agency for Technology and Innovation (TEKES) grant No. 2243/31/05. Wihuri Research Institute is maintained by Jenny and Antti Wihuri Foundation
Correspondence to: Anna Oksaharju, MSc, Wihuri Research Institute, Kalliolinnantie 4, Helsinki 00140, Finland. anna.oksaharju@helsinki.fi
Telephone: +358-9-681411 Fax: +358-9-637476
Received: May 14, 2010 Revised: August 28, 2010 Accepted: September 5, 2010 Published online: February 14, 2011
Abstract
AIM: To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.
METHODS: Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L. rhamnosus) GG (LGG®), L. rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS) and Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination for 3 or 24 h, and were subjected to global microarray analysis using an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.
RESULTS: LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G, respectively) and histamine H4 receptor. LGG, Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation, and on several genes that encoded proteins with a pro-inflammatory impact, such as interleukin (IL)-8 and tumour necrosis factor alpha. Also genes that encoded proteins with anti-inflammatory functions, such as IL-10, were upregulated.
CONCLUSION: Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes, and by inducing a pro-inflammatory response.
