Wang L, Li CL, Wang L, Yu WB, Yin HP, Zhang GY, Zhang LF, Li S, Hu SY. Influence of CXCR4/SDF-1 axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells. World J Gastroenterol 2011; 17(5): 625-632 [PMID: 21350711 DOI: 10.3748/wjg.v17.i5.625]
Corresponding Author of This Article
San-Yuan Hu, MD, Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China. 33943219@qq.com
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Original Article
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World J Gastroenterol. Feb 7, 2011; 17(5): 625-632 Published online Feb 7, 2011. doi: 10.3748/wjg.v17.i5.625
Influence of CXCR4/SDF-1 axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells
Lin Wang, Cui-Ling Li, Lei Wang, Wen-Bin Yu, Hai-Peng Yin, Guang-Yong Zhang, Li-Feng Zhang, Sheng Li, San-Yuan Hu
Lin Wang, Lei Wang, Wen-Bin Yu, Guang-Yong Zhang, Li-Feng Zhang, San-Yuan Hu, Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Cui-Ling Li, Hai-Peng Yin, Key Laboratory for Modern Medicine and Technology of Shandong Province, Institute of Basic Medicines, Shandong Academy of Medical Sciences, Jinan 250062, Shandong Province, China
Sheng Li, Department of Hepatobiliary Surgery, Shandong Tumor Hospital, Jinan 250117, Shandong Province, China
Author contributions: Hu SY and Li S designed the study; Li CL, Yin HP, Zhang LF and Wang L performed the experiments; Wang L, Yu WB and Zhang GY analyzed the data; Wang L wrote the paper.
Supported by National Natural Science Foundation of China, No. 30571712 and 30810403081
Correspondence to: San-Yuan Hu, MD, Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China. 33943219@qq.com
Telephone: +86-531-82169441 Fax: +86-531-82169441
Received: October 15, 2010 Revised: January 11, 2011 Accepted: January 18, 2011 Published online: February 7, 2011
Abstract
AIM: To study the influence of CXCR4/stromal cell-derived factor-1 (SDF-1) axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells and its underlying mechanisms.
METHODS: Effect of SDF-1 on E-cadherin/β-catenin expression was detected by immunocytochemistry. E-cadherin and β-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction. SDF-1-induced phosphorylation of phosphatidylinositol 3-kinase (PI3K)/AKT and β-catenin was detected by Western blotting.
RESULTS: The E-cadherin and β-catenin mRNA expression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL (P < 0.05). SDF-1-induced significant phosphorylation of PI3K/AKT and β-catenin. AMD3100 and LY294002 inhibited the phosphorylation of PI3K/AKT and β-catenin.
CONCLUSION: SDF-1 down-regulates the E-cadherin/β-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing β-catenin phosphorylation.
