Benyair R, Kondratyev M, Veselkin E, Tolchinsky S, Shenkman M, Lurie Y, Lederkremer GZ. Constant serum levels of secreted asialoglycoprotein receptor sH2a and decrease with cirrhosis. World J Gastroenterol 2011; 17(48): 5305-5309 [PMID: 22219600 DOI: 10.3748/wjg.v17.i48.5305]
Corresponding Author of This Article
Dr. Gerardo Z Lederkremer, Departments of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel. gerardo@post.tau.ac.il
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Brief Article
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World J Gastroenterol. Dec 28, 2011; 17(48): 5305-5309 Published online Dec 28, 2011. doi: 10.3748/wjg.v17.i48.5305
Constant serum levels of secreted asialoglycoprotein receptor sH2a and decrease with cirrhosis
Ron Benyair, Maria Kondratyev, Elena Veselkin, Sandra Tolchinsky, Marina Shenkman, Yoav Lurie, Gerardo Z Lederkremer
Ron Benyair, Maria Kondratyev, Elena Veselkin, Sandra Tolchinsky, Marina Shenkman, Gerardo Z Lederkremer, Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Yoav Lurie, Liver Unit, Department of Gastroenterology, Tel Aviv Sourasky Medical Center, Tel Aviv 69978, Israel
Author contributions: Benyair R and Kondratyev M contributed equally to this work; Lurie Y and Lederkremer GZ designed the research; Benyair R, Kondratyev M, Veselkin E, Tolchinsky S and Shenkman M performed the research; Lurie Y contributed study samples; Benyair R, Kondratyev M and Lederkremer GZ analyzed the data; and Lederkremer GZ wrote the manuscript.
Supported by The Ramot research fund, the Israel Science Foundation, the Israel Cancer Association and the Israeli Ministry of Health (to Lederkremer GZ)
Correspondence to: Dr. Gerardo Z Lederkremer, Departments of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel. gerardo@post.tau.ac.il
Telephone: +972-3-6409239 Fax: +972-3-6422046
Received: May 20, 2011 Revised: June 28, 2011 Accepted: July 11, 2011 Published online: December 28, 2011
Abstract
AIM: To investigate the existence and levels of sH2a, a soluble secreted form of the asialoglycoprotein receptor in human serum.
METHODS: Production of recombinant sH2a and development of a monoclonal antibody and an enzyme-linked immunosorbent assay (ELISA). This assay was used to determine the presence and concentration of sH2a in human sera of individuals of both sexes and a wide range of ages.
RESULTS: The recombinant protein was produced successfully and a specific ELISA assay was developed. The levels of sH2a in sera from 62 healthy individuals varied minimally (147 ± 19 ng/mL). In contrast, 5 hepatitis C patients with cirrhosis showed much decreased sH2a levels (50 ± 9 ng/mL).
CONCLUSION: Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals. This constant level and the decrease with cirrhosis suggest a diagnostic potential.
